We narrowed to 11,549 results for: phen
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Plasmid#159099PurposeCckA FRET Sensor with DITE motif mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
ExpressionBacterialMutationCckA D293 mutated to A293 and V199I on mRuby3 (P…PromoterXyloseAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE669
Plasmid#153235PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
TagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7Available SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE672
Plasmid#153238PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE473
Plasmid#153264PurposeM1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE500
Plasmid#153273PurposeM6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE502
Plasmid#153275PurposeM8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
C335
Plasmid#141125PurposeExpresses chimeric repressor (RbsR-L) under Cin promoterDepositorInsertsTagsssrA degradation tagExpressionBacterialMutationChimera: DNA binding domain of LacI and ligand bi…Promoterengineered cin promoter (responds to C14-HSL) and…Available SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMT201
Plasmid#134816PurposeSMT101 with TET promoter for TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
ExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP35-TD-HilA
Plasmid#153332PurposeTriggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinoseDepositorInsertHA4-7c12 Tandem Monobody
UseSalmonella expressionAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP92-TD-HilA
Plasmid#153331PurposeTriggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinoseDepositorInsertSptP92-HA4-7c12 Tandem Monobody
UseSalmonella expressionAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-3G124-HilA
Plasmid#153324PurposeShows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vectorDepositorInsert3G124 DARPin but no expression
UseSalmonella expressionAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046774
Plasmid#145574PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046846
Plasmid#145575PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MYMH 351
Plasmid#138058PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-10AG 11AC-GFP
ExpressionBacterialMutationAAA GAG GAG GCAAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 336
Plasmid#138057PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 2AC 11AC-GFP
ExpressionBacterialMutationGCA GAG GAG ACAAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 333
Plasmid#138056PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 11AC-GFP
ExpressionBacterialMutationGAA GAG GAG ACAAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 330
Plasmid#138055PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb- 2AC 11AC-GFP
ExpressionBacterialMutationACA GAG GAG ACAAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 327
Plasmid#138054PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 2AC-GFP
ExpressionBacterialMutationGCA GAG GAG AAAAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK110
Plasmid#110576PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP6 Promoter with RBS
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK112
Plasmid#110577PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP12b Promoter with RBS
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK113
Plasmid#110578PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP32 Promoter with RBS
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MTK234_005
Plasmid#123992PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA homology arm-1)
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_006
Plasmid#123993PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA Homology arm-2
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_007
Plasmid#123994PurposeEncodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA for cROSA Homology arm-3
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_056
Plasmid#123921PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-hu6_20N_cr3
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_026
Plasmid#123906PurposeEncodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInserthuman non targeting sgRNA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_028
Plasmid#123908PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hThal5.10-2_hg18
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_029
Plasmid#123909PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hThal5.10-2_hg18
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_013
Plasmid#123867PurposeEncodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK systemDepositorInsertWPRE-Blast (no terminator)
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_018
Plasmid#123733PurposeEncodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-frb-D10A-ERT2
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG
Plasmid#130684PurposeExpresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
Tags3xFLAGExpressionMammalianMutationD453_F489del, E653_G1960delPromoterCMVAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG
Plasmid#130683PurposeExpresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
Tags3xFLAGExpressionMammalianMutationD409_E452del, E653_G1960delPromoterCMVAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-4xUAS
Plasmid#125149PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
JK305: pMVP/Blast-DEST
Plasmid#121855PurposepMVP destination vector, empty expression vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JL104: pMVP/Neo-DEST
Plasmid#121857PurposepMVP destination vector, empty expression vector backbone w/ Neo selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD250.2
Plasmid#125077PurposeExpression of torR under PLtetO-1, output promoter PtorCAD129DepositorAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR300.3
Plasmid#125079PurposeExpression of uhpA under PLtetO-1, output Promoter PuhpT99DepositorAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(F317D, I325A)
Plasmid#122275PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317H)
Plasmid#122269PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to HistidineAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D, I325A)
Plasmid#122273PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…Available SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317Q)
Plasmid#122271PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to glutamineAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D)
Plasmid#121391PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterADH1 promoterAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317G)
Plasmid#122268PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to glycineAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317K)
Plasmid#122270PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to lysineAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(F317D)
Plasmid#121396PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterADH1 promoterAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA-YFP-R5
Plasmid#106257PurposeExpresses Venus YFP fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertYellow fluorescent protein
TagsAdded silaffin R5 peptide to C terminus of YFP af…ExpressionBacterialPromoterpL-TetOAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPB1–4stop
Plasmid#112824PurposepSMF2 with repeats 8–10 interrupted with 4 repeats containing stop codonsDepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationpSMF2 with repeats 8–10 interrupted with 4 repeat…PromoterNative promoterAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys
Plasmid#110541PurposeBacterial protein of E.coli DHFR C85A/C152S mutantDepositorInsertecDHFR
ExpressionBacterialMutationC85 mutated to A85, C152 mutated to S152PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only