We narrowed to 14,321 results for: cas9

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

Plasmid

#159663

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

Plasmid

#159662

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

Plasmid

#159656

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Publication

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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SauCas9 PE2*

Plasmid

#169851

Purpose

Mammalian Expression, sauCas9 based prime editor

Depositor

Scot Wolfe

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SaCas9-N580A-M-MLV

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Promoter

cmv

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g5

Plasmid

#80788

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

Depositor

Yonglun Luo

Publication

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3 CAS9 IRES-Dsred

Plasmid

#129444

Purpose

expression of FnCas9 and DsRed

Depositor

Oskar Laur

Insert

FnCas9 IRES-Dsred

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-p300_Blast

Plasmid

#192654

Purpose

3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Publication

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9_human_ADAR1_ccB

Plasmid

#158116

Purpose

deletion hADAR1

Depositor

Emanuela Felley-Bosco

Insert

Adar1 (ADAR Human)

Expression

Mammalian

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmiHiFiCas9

Plasmid

#164832

Purpose

miHiFiCas9 expressing plasmid

Depositor

Eugene Chen

Publication

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miHiFiCas9 (contains Brex27 at sequence 5031-5138).

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

change R 691 to A

Promoter

CMV

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEF_dCas9-VP64

Plasmid

#68417

Purpose

Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG and VP64

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

Plasmid

#195502

Purpose

Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

Depositor

Alexander Meissner

Publication

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

U6

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

Plasmid

#117325

Purpose

CRISPR-Cas9 for miR-124-2/3, 3'

Depositor

Volker Busskamp

Publication

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-2/3-3' (MIR124-3 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

Plasmid

#117324

Purpose

CRISPR-Cas9 for miR-124-1, 3'

Depositor

Volker Busskamp

Publication

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-1-3' (MIR124-1 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

Plasmid

#117323

Purpose

CRISPR-Cas9 for miR-124-3, 5'

Depositor

Volker Busskamp

Publication

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-3-5' (MIR124-3 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

Plasmid

#117322

Purpose

CRISPR-Cas9 for miR-124-1/2, 5'

Depositor

Volker Busskamp

Publication

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-1/2-5' (MIR124-1 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

Plasmid

#91884

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

Plasmid

#91877

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

Plasmid

#91883

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Publication

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only