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Showing: 6211 - 6240 of 165703 results

  6. AICSDP-8:TOMM20-mEGFP

    Plasmid
    #87423
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87423-plasmid-map-sequence-id-173982

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  7. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  9. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  17. pAAV-synP-FLEX-splitTVA-EGFP-B19G (AAV1)

    Viral Prep
    #52473-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-synP-FLEX-splitTVA-EGFP-B19G (#52473). In addition to the viral particles, you will also receive purified pAAV-synP-FLEX-splitTVA-EGFP-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Article
    Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (AAV1)

    Viral Prep
    #68719-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (#68719). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor with bicistronic mRuby2. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Promoter
    CAG-FLEX
    Tags
    mRuby2 (Cre-dependent)
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. EF1a mCherry

    Plasmid
    #129431
    Purpose
    expression of mCherry from EF1a promoter
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129431-plasmid-map-sequence-id-256111

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  21. R777-E283 Hs.ROCK1

    Plasmid
    #70567
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70567-plasmid-map-sequence-id-126016

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  24. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

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Showing: 6211 - 6240 of 165703 results