We narrowed to 5,560 results for: SPI

1. CKAP2 pHAT

   Plasmid

   #175946

   Purpose

   Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

   Depositor

   Susanne Bechstedt

   Article

   McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

   Insert

   CKAP2 (Ckap2 Mouse)
   

   Use

   Tags

   6-His and StrepTagII

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHelA12

   Plasmid

   #176493

   Purpose

   Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

   Depositor

   Jian-Dong Huang, Xiaodong Cheng

   Article

   Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

   Insert

   SARS-CoV NSP13 (ORF1ab SARS-CoV)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   V570I

   Promoter

   T7

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

   Plasmid

   #160610

   Purpose

   Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:AsCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Venus-G protein Gamma 5

   Plasmid

   #42200

   Depositor

   Narasimhan Gautam

   Article

   Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

   Insert

   Gamma 5 (Gng5 Rat)
   

   Use

   Tags

   Venus(1-155)

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   CMV

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

5. R9-WT

   Plasmid

   #125210

   Purpose

   Expressing wildtype human Rad9b gene

   Depositor

   Richard Finnell

   Article

   Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

   Insert

   Rad9b (RAD9B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 8200 Bicistronic_GFP_ires_hygro

   Plasmid

   #64375

   Purpose

   This a retroviral expression plasmid expressing GFP along with hygro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMT-Tyrosinase-bait-T2a-Cherry-pA

   Plasmid

   #111155

   Purpose

   knock in bait for Axolotl Tyrosinase locus

   Depositor

   Elly Tanaka

   Article

   Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

   Insert

   Tyrosinase-bait-T2a-Cherry-pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

   Plasmid

   #172317

   Purpose

   CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJS278

   Plasmid

   #134139

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa 132-255, R155A

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST

    Plasmid

    #175018

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    OK-HEF s57a ED
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    s57a esterase knock out mutant

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST15 hSlp4-a

    Plasmid

    #40046

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a (SYTL4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-dLbCpf1-BE0ΔiNLS

    Plasmid

    #107684

    Purpose

    Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0ΔiNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDK480

    Plasmid

    #134197

    Purpose

    Mammalian expression of Flip in SKAP and IRES GFP

    Depositor

    Iain Cheeseman

    Article

    Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

    Insert

    untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Alternate Start, G5 siRNA hardened

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRVH1-hygro shStx3

    Plasmid

    #40071

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Stx3 shRNA-1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCRII-Topo Diap3 in situ probe

    Plasmid

    #45602

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    Diap3 in situ probe (Diaph3 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 1589-2321 of BC028920

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCRII-Topo Crim1 in situ probe

    Plasmid

    #45600

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    Crim1 in situ probe (Crim1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    insert contains bp#4062-4502 of NM_015800.3

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pRSF-G1(7CNW)RS

    Plasmid

    #222874

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tom20-ECFP-FRB

    Plasmid

    #210289

    Purpose

    mitochondrial OM marker and "anchor" in FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    Tom20-ECFP-FRB
    

    Use

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ECFP-FRB-Sec61b

    Plasmid

    #210290

    Purpose

    nucleus OM marker and "anchor" for FKBP-FRB CID

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    ECFP-FRB-Sec61b
    

    Use

    Tags

    ECFP and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tom20-EYFP-miLID

    Plasmid

    #210291

    Purpose

    mitochondria marker and "anchor" for optogenetics

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

    Insert

    Tom20-EYFP-miLID
    

    Use

    Tags

    EYFP and iLID

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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