We narrowed to 8,463 results for: gnal

1. Flag‐mTim3 (del.Cyto)

   Plasmid

   #49209

   Purpose

   expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 (del.Cyto) (Havcr2 Mouse)
   

   Tags

   CD8 signal sequence and FLAG

   Expression

   Mammalian

   Mutation

   cytosolic domain deleted, no Tim3 signal sequence…

   Promoter

   Ef1a

   Available Since

   Dec. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag‐mTim3 (T2 truncation)

   Plasmid

   #49211

   Purpose

   expresses Flag tagged mouse Tim3 (T2 truncation)

   Depositor

   Lawrence Kane

   Article

   Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

   Insert

   Tim3 T2 truncation (Havcr2 Mouse)
   

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   T2 truncation ( lacks all but one tyrosine, which…

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_dCas9-KRAB_sgSTK3i_#2 (PuroR)

   Plasmid

   #209772

   Purpose

   CRISPRi for STK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK3i (STK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_dCas9-KRAB_sgSTK3i_#1 (PuroR)

   Plasmid

   #209771

   Purpose

   CRISPRi for STK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK3i (STK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBpuro-EGFP-eDHFR(69K6)-cRaf

   Plasmid

   #178849

   Purpose

   Mammalian expression of cRaf fused to EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-cRaf (RAF1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBpuro-cRaf-mNG-eDHFR(69K6)

   Plasmid

   #172107

   Purpose

   Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

   Plasmid

   #62580

   Purpose

   Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Mutation

   miR-21 binding site 1 mutant (TAAGCTA --> TAGA…

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

   Plasmid

   #62581

   Purpose

   Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Mutation

   miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

9. GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

   Plasmid

   #78507

   Purpose

   Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   Gads SH3 SH2 SH3 domain (GRAP2 Human)
   

   Tags

   GST followed by a prescission protease claevage s…

   Expression

   Bacterial

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGem-sHyper:sAPX

    Plasmid

    #84738

    Purpose

    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Promoter

    pFMV/p35S

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGem-nHyPer:sAPX

    Plasmid

    #84735

    Purpose

    Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    nHyper (HyPer2 targeted to nucleus)
    

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Promoter

    FMV/35S

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBridge-tyrosinase.σ1A

    Plasmid

    #197381

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-1 σ1A
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and SV4…

    Expression

    Yeast

    Mutation

    Contains an extra 42 nucleotides encoding 14 resi…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)

    Plasmid

    #209775

    Purpose

    CRISPRi for STK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    dCas9-Sniper-KRAB, sgSTK3i (STK3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIR-Report-RASA1-3'UTR

    Plasmid

    #62575

    Purpose

    Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    RASA1 (RASA1 Human)
    

    Use

    Luciferase

    Tags

    None

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

    Plasmid

    #178854

    Purpose

    Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
    

    Expression

    Mammalian

    Mutation

    Grb2: Y160E

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

    Plasmid

    #172105

    Purpose

    Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    miRFP670-eDHFR(69K6)-Gαs (GNAS Human)
    

    Expression

    Mammalian

    Mutation

    Gαs: C3S

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid

    #236230

    Purpose

    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    

    Use

    AAV

    Tags

    mEmerald

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF2-EFGR-hMer

    Plasmid

    #225996

    Purpose

    Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

    Insert

    human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)
    

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIL6-PMEL-hbb

    Plasmid

    #239868

    Purpose

    Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.

    Depositor

    Catherine Jopling

    Article

    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

    Insert

    Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    

    Expression

    Mammalian

    Mutation

    Valine to Glycine change is at amino acid 26 of n…

    Promoter

    T7 (included as part of insert)

    Available Since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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