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Plasmid#79997PurposeClathrin labeling with near-infrared fluorescent protein miRFP703DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClathrin (CLTB Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-85b
Plasmid#53282PurposeContains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthaseDepositorInsertcrtE, crtY, crtI
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)
Plasmid#55221DepositorTypeEmpty backboneTagsStrepII and msfGFPExpressionInsectAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGs-NLS-TIR1_P2A_NES-TIR1
Plasmid#117699PurposeExpression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNADepositorInsertsTRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1
TagsHA tag, Myc-NES, and NLSExpressionMammalianMutationcodon optimized for murine expressionPromoterNone, 2A peptide from porcine teschovirus-1 polyp…Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-CAD
Plasmid#73566PurposeSoluble red fluorescent activator of Orai1 channelsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)
Tags6x His and mCherryExpressionMammalianPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
psiCHECK-2-TGFBR2-wt_3'UTR
Plasmid#31882DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTGFBR2 wild-type 3'UTR (TGFBR2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterSV40Available sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-U6-pegSERPINA1-U6-Nicking-PE2-N
Plasmid#164907PurposeExpress a pegRNA targeting SERPINA1, and a nicking sgRNA and the N-terminal split-intein fragment of PE2DepositorInsertpegSERPINA1, Nicking sgRNA, PE2-N
UseAAVAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
TagsHis tag and LAA degradation tagExpressionBacterialAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
LeGO-Cer
Plasmid#27351DepositorPublicationInsertU6 promoter, SFFV promoter, Cerulean
UseLentiviralExpressionMammalianAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mApple-TNFa-RUSH
Plasmid#166902PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mAppleDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmApple
ExpressionMammalianAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTC-ApoE-Tet
Plasmid#85578PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liverDepositorInsertCreER
UseCre/LoxExpressionMammalianPromoterApoE.HCR.hAATAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-PesaRlux
Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorPublicationInsertPesaR-luxCDABE-term
UseSynthetic BiologyExpressionBacterialPromoterPesaRAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-Pur-CAG-hM4Di-mCherry
Plasmid#80947Purposehuman AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthM4Di-mCherry
Tagsmcherry fusionExpressionMammalianPromoterCAG promoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPR219.05
Plasmid#78554PurposePlasmid expressing UirR, response regulator and PcsiR1-regulated GFP outputDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertuirR
UseSynthetic BiologyPromoterJ23115Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hygro-iDEST
Plasmid#75339PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
ExpressionMammalianPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKII-Archon1-EGFP
Plasmid#108417PurposeAAV production plasmid encoding for Archon1 fluorescent voltage reporterDepositorInsertArchon1-EGFP
UseAAVExpressionMammalianPromoterCaMKIIAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJZC620
Plasmid#62282PurposeExpresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cellsDepositorInsertsMCP-VP64
PCP-VP64
dCas9
TagsVP64ExpressionYeastMutationNuclease activity has been inactivated by mutatio…PromoterpAdh and pTdh3Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPtGE34
Plasmid#107932PurposeExpresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
UseCRISPR and Synthetic Biology; Episomal vector for…Available sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B
Pooled library#92380PurposeCRISPRaDepositorUseLentiviralAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by