We narrowed to 39,378 results for: Vit;

1. pAAV-pTH-iCre:EGFP-WPREpA

   Plasmid

   #213142

   Purpose

   Truncated rat TH promoter expressing iCre fused to EGFP

   Depositor

   Ulrik Gether

   Article

   Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

   Insert

   iCre
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated TH promoter from rat (Rattus norvegicus…

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-HA-GOLPH3

   Plasmid

   #21687

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-fDIO-tdTomato

   Plasmid

   #128434

   Purpose

   Expresses tdTomato under control of eukaryotic Ef1a promoter after Flp-dependent recombination

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Has Service

   AAV1

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1_oROS-HT

   Plasmid

   #216413

   Purpose

   Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SspB-HaloTag-TIAM1-DHPH

   Plasmid

   #176127

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-FLAG-APEX2-LGALS3

   Plasmid

   #175756

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

   Plasmid

   #171027

   Purpose

   Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Has Service

   AAV1 and AAV9

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   ChrimsonR is fused to tdTomato

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX/PD-L1 WT

   Plasmid

   #121466

   Purpose

   Expression of GST-human PD-L1 in E. Coli

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. STARR-seq luciferase validation vector_ORI_neg.cont

   Plasmid

   #99315

   Purpose

   Negative control for luciferase validation vectors

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   neutral region; chr3: 55198740-55200041
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-5X-1-LARP7

    Plasmid

    #113545

    Purpose

    Produces an N-terminal GST-tagged full length LARP7 protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    LARP7 (LARP7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRES2-hPAC-FLAG

    Plasmid

    #129494

    Purpose

    Expresses human TMEM206 tagged at the C-terminus with FLAG

    Depositor

    Zhaozhu Qiu

    Article

    Yang et al Science. 2019 Apr 26;364(6438):395-399.

    Insert

    human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPHK2 gRNA (BRDN0001148465)

    Plasmid

    #77093

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

    Plasmid

    #112008

    Purpose

    For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Insert

    axon-GCaMP6s-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPHK1 gRNA (BRDN0001147454)

    Plasmid

    #76813

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet-1 Hepatitis C Virus NS3/NS4A

    Plasmid

    #61696

    Purpose

    inducible dual expression of NS3 protease along with its cofactor NS4A

    Depositor

    Michael Johnson

    Article

    Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

    Inserts

    NS4A
    NS3
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. non-targeting control gRNA (BRDN0001145694)

    Plasmid

    #80191

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. S1PR1-DuET

    Plasmid

    #213374

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    S1PR1 (S1PR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEM-Cas9HF1

    Plasmid

    #89961

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9HF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. araBAD-BCD20-ClpP-BCD17-ClpX

    Plasmid

    #153301

    Purpose

    Expresses clpX and clpP in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Inserts

    clpX (clpX Escherichia coli)
    clpP (clpP Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV3Tag8li-hADARB2-3xFLAG

    Plasmid

    #165470

    Purpose

    Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRetroX-TRE3G_NT-GSDMD

    Plasmid

    #201170

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only

    Promoter

    TRE3G

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. p5E--3mnx1

    Plasmid

    #74632

    Purpose

    Can be used to drive expression specifically in zebrafish motor neurons

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX-CMV-Galpha-oA ONE-GO

    Plasmid

    #189732

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oA ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    I250 (silent mutation in YFP)

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. non-targeting control gRNA (BRDN0001148862)

    Plasmid

    #80263

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. WN10150

    Plasmid

    #80443

    Purpose

    Expresses dead (908A) AsCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    nuclease dead (908A) AsCpf1
    

    Use

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    nuclease dead (D908A)

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 608 pSG5L HA RB del ex4

    Plasmid

    #10730

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del exon4 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of exon4

    Promoter

    Available since

    Oct. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

28. 409 pSG5L HA RB (379-928)

    Plasmid

    #10734

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB (379-928) (RB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 379-928

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

29. pAAV-Ef1a-fDIO-GCaMP6f

    Plasmid

    #128315

    Purpose

    Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-CMV-Galpha-13 ONE-GO

    Plasmid

    #189740

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-13 ONE-GO
    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L342M (present in YFP but no functional consequen…

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CMV-YE1-BE3-FNLS-CMV-mCherry

    Plasmid

    #154005

    Purpose

    Express YE1-BE3-FNLS

    Depositor

    Hui Yang

    Article

    Zuo et al Nat Methods. 2020 May 18. pii: 10.1038/s

    Insert

    YE1-BE3-FNLS
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 498 pSG5L HA RB del663 (NAAIRS)

    Plasmid

    #10728

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del663 (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 664 to 669 were replaced by NAAIRS, a fl…

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

33. pAAV.Syn.NES-jRGECO1b.WPRE.SV40

    Plasmid

    #100857

    Purpose

    AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFBD-Akt13C(S473D)

    Plasmid

    #86577

    Purpose

    Expresses cleavable human full-length Akt1(S473D) phosphomimetic

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130; S473D phos…

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-hsyn-GRAB_rDA-mut

    Plasmid

    #140558

    Purpose

    Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ABE8.20-d

    Plasmid

    #136301

    Purpose

    expresses ABE8.20-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG mEmerald-EB3

    Plasmid

    #168514

    Purpose

    Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pWPXL-c-Myc

    Plasmid

    #36980

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pLentiPGK Puro DEST JNKKTRmCerulean3

    Plasmid

    #90232

    Purpose

    Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge