We narrowed to 15,991 results for: LEA

1. pSGKP_AcsgRNA

   Plasmid

   #203807

   Purpose

   pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcgRNA
   

   Use

   CRISPR

   Promoter

   J23119

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SpRY

   Plasmid

   #198544

   Purpose

   Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 FLIPE-1m

   Plasmid

   #13544

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207W
   

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207W in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-ninein coiled-coil 2

   Plasmid

   #73522

   Purpose

   Encodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

   Depositor

   Michel Bornens

   Article

   Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

   Insert

   ninein coiled-coil 2 (Nin Mouse)
   

   Tags

   EGFP tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pEGFP-ninein coiled-coil 1

   Plasmid

   #73521

   Purpose

   Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

   Depositor

   Michel Bornens

   Article

   Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

   Insert

   ninein coiled-coil 1 (Nin Mouse)
   

   Tags

   EGFP tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

6. p2T-CMV-eA3Amax-T31A-NG-BlastR

   Plasmid

   #153000

   Purpose

   C-to-T/G/A base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   eA3A-BE4 T31A-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YTIP-GFP

   Plasmid

   #42238

   Depositor

   William Pu

   Article

   von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

   Insert

   YTIP-GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-ERK2 T183AY185F

   Plasmid

   #118299

   Purpose

   Expression of Rat ERK2

   Depositor

   Philip Stork

   Article

   Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

   Insert

   ERK2 T183AY185F (Mapk1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T183AY185F

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSC236

   Plasmid

   #216368

   Purpose

   xhNup155-Nb2i (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup155-Nb2i (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSC235

    Plasmid

    #216372

    Purpose

    xhNup155-Nb3i (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb3i (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSC227

    Plasmid

    #216351

    Purpose

    xhNup35-Nb1t (+3Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup35-Nb1t (+3Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3xEGFP-mMeikin

    Plasmid

    #174708

    Purpose

    Mammalian expression of mouse Meikin

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin (Meikin Mouse)
    

    Tags

    3xEGFP

    Expression

    Mammalian

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-TetO(3G)-WPRE

    Plasmid

    #186206

    Purpose

    tTA dependent gene expression vector

    Depositor

    Naoshige Uchida

    Article

    Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

    Type

    Empty backbone

    Use

    AAV

    Promoter

    TetO(3G)

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-HA-GlpG

    Plasmid

    #155135

    Purpose

    Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-flag-mKdm4d(H189A)-polyA

    Plasmid

    #61554

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (catalytic mutant)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    changed Histidine 189 (of Kdm4d) to Alanine

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTYF-sGFAP-IRES-tdTomato

    Plasmid

    #166920

    Purpose

    Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    sGFAP

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KBB364

    Plasmid

    #185083

    Purpose

    RFA1 fused to GFP under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLSU4cg10

    Plasmid

    #126078

    Purpose

    Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Promoter

    longCaMV35S

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTG131

    Plasmid

    #198640

    Purpose

    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    Cas9
    

    Expression

    Bacterial

    Promoter

    pPhlF

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMJ839

    Plasmid

    #39317

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (NMA0631 Neisseria meningitidis)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only