We narrowed to 7,284 results for: Ank

1. pMMS22L-siR3

   Plasmid

   #221013

   Purpose

   mammalian expression vector of myc-Flag tagged MMS22L siRNA resistant

   Depositor

   Daniel Durocher

   Article

   O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

   Insert

   MMS22L (MMS22L Human)
   

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   silent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…

   Promoter

   CMV

   Available Since

   July 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLVX-Flag-CDK19-WT-IRES-ZsGreen

   Plasmid

   #68858

   Purpose

   Expresses Human CDK19 Wild-Type

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

   Plasmid

   #103869

   Purpose

   dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-eGFP-SHLD1

   Plasmid

   #114116

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD1

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD1 (SHLD1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFB-EPCP1

   Plasmid

   #226783

   Purpose

   Expression of Z-tagged LRRK2 RCKW using Baculoviruses

   Depositor

   Sebastian Mathea

   Article

   Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Tags

   His-Z-TEV

   Expression

   Insect

   Mutation

   Amino acids deleted 1-1326

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINTO-N3::hTET3 HxD

   Plasmid

   #136365

   Purpose

   Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Tags

   2xStrepTagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   HxD (H1077Y, D1079A)

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW-eGFP-SHLD2

   Plasmid

   #114119

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (SHLD2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-hSyn-JEDI-2P-Kv-WPRE

   Plasmid

   #179463

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Has Service

   AAV9

   Insert

   JEDI-2P-Kv
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0047-CMV-dPspCas13b-ADAR1DD(E1008Q)

   Plasmid

   #103863

   Purpose

   dPspCas13b-ADAR1DD(E1008Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR1DD (ADAR Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-eGFP-SHLD3

    Plasmid

    #114126

    Purpose

    Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMSCVpuro-Mito-DsRed1

    Plasmid

    #87379

    Purpose

    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

    Depositor

    Björn Stork

    Article

    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

    Insert

    TXN2 mitochondrial transit peptide (TXN2 Human)
    

    Use

    Retroviral

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    First 60 aa only

    Promoter

    Retroviral 5' LTR promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-AlkB

    Plasmid

    #228218

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Junchao Shi

    Article

    Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

    Insert

    Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO-PPO-Venus

    Plasmid

    #139505

    Purpose

    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Has Service

    AAV8 and AAV9

    Insert

    parapinopsin
    

    Use

    AAV

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKIIa-JEDI-2P-Kv-WPRE

    Plasmid

    #179465

    Purpose

    Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P-Kv
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-Flag-CDK8-W105M-IRES-mCherry

    Plasmid

    #68857

    Purpose

    Expresses Human CDK8 W105M Mutant

    Depositor

    Matthew Shair

    Article

    Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

    Insert

    Cyclin-Dependent Kinase 8 (CDK8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    W105M

    Promoter

    EF1α

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCJ208

    Plasmid

    #162681

    Purpose

    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Replacement of W185-F191 with G251-T472 from MHET…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lucMAPT-30D

    Plasmid

    #214672

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. lucMAPT-30U

    Plasmid

    #214673

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGFP-mHP1beta

    Plasmid

    #181901

    Purpose

    Fluorescently tagged HP1beta

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1beta (Cbx1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT/TO-eGFP-SHLD3

    Plasmid

    #114125

    Purpose

    Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV/TetO2

    Available Since

    Aug. 16, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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