We narrowed to 22,487 results for: SCR

1. pGEM-SNAP-TRIM21

   Plasmid

   #196181

   Purpose

   For in vitro transcription of SNAP-TRIM21 mRNA

   Depositor

   Binyam Mogessie

   Article

   Dunkley et al Sci Adv. 2023 Jan 20;9(3):eadc9161. doi:

   Insert

   SNAP-TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH_35S/mEGFP

   Plasmid

   #135321

   Purpose

   mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

   Depositor

   Mathias Pribil

   Article

   Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBRα

   Plasmid

   #53731

   Purpose

   Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   αNTD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 CDK8 HA (P#634)

   Plasmid

   #14649

   Depositor

   Matija Peterlin

   Article

   Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

   Insert

   Cdk8 (CDK8 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-GATA3

   Plasmid

   #1332

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Science 2000 Oct 6;290(5489):134-8.

   Insert

   GATA3 (Gata3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-AaCas12b

   Plasmid

   #113433

   Purpose

   Expresses Expresses 10xHis-MBP fused to AaCas12b

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AaCas12b
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJZC522

   Plasmid

   #62279

   Purpose

   MCP-VP64 fusion

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   MCP-VP64
   

   Use

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Promoter

   pAdh

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSP561

   Plasmid

   #139497

   Purpose

   Expression vector for the MS2-GFP protein in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pADH1 MS2-GFPenvy tCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ∆FG

   Promoter

   pADH1

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHBV-S1-Luc

   Plasmid

   #71416

   Purpose

   To see S1 promoter activity of HBV for luciferase assay

   Depositor

   Wang-Shick Ryu

   Article

   Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

   Insert

   HBV S1 promoter driving Luciferase expression
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HBV S1

   Available since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHBV-S2-Luc

    Plasmid

    #71417

    Purpose

    To see HBV S2 promoter activity for luciferase assay

    Depositor

    Wang-Shick Ryu

    Article

    Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

    Insert

    HBV S2 promoter driving luciferase expression
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HBV S2

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-mBrn2

    Plasmid

    #19711

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    mouse Brn2 (Pou3f2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-4xnrUAS

    Plasmid

    #87120

    Purpose

    Multisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elements

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    4xnrUAS (non repetitive)
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. epB_CAG_TetRFlag-nls-tdTom_DEx4

    Plasmid

    #119909

    Purpose

    ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    TetRFlag-nls-tdTom
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p70a-T7RNAP

    Plasmid

    #105683

    Purpose

    Expresses T7 polymerase in cell-free TXTL.

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTomatoSlicer

    Plasmid

    #221085

    Purpose

    Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-T3G-CERP2

    Plasmid

    #175498

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pxpr_502-wt-ltr

    Plasmid

    #182647

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Use

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-mKate2-NS

    Plasmid

    #109729

    Purpose

    Multisite gateway vector for 5' tagging with mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV5-CBFbeta

    Plasmid

    #12427

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    CBF beta (Cbfb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

21. YFP-DBD

    Plasmid

    #14874

    Depositor

    Alexandra Newton

    Article

    Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

    Insert

    DAG binding domain (Prkcb Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Y123W

    Promoter

    Available since

    Aug. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pXPR_124

    Plasmid

    #133461

    Purpose

    EF1a promoter expresses SpyoCas9 and P2A provides EGFP

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    SpyoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCarmine in pRSETB

    Plasmid

    #109484

    Purpose

    bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

    Depositor

    Oliver Griesbeck

    Article

    Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

    Insert

    mCarmine
    

    Use

    Tags

    N-terminal histidine tag in vector

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRDA_449

    Plasmid

    #179093

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA/Myc DeltaN TCF4

    Plasmid

    #16513

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta N: lacks aa 1-32

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pAAV-beta-arrestin2-HA-TEV219

    Plasmid

    #104845

    Purpose

    b2AR-SPARK protease component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    beta-arrestin2-HA-TEV219
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETM6-mCherry

    Plasmid

    #66534

    Purpose

    Expresses mCherry under the control of the Concensus T7 Promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. T. aq RNA Polymerase

    Plasmid

    #215349

    Purpose

    Express T. aq RNA Polymerase subunits in E. coli cells

    Depositor

    Julius Lucks

    Article

    Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

    Insert

    RNA polymerase
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUHD-AID

    Plasmid

    #121066

    Purpose

    vector expresses AID-fusion protein under TRE promoter and with puromycin resistance selection

    Depositor

    Randy Poon

    Article

    Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

    Type

    Empty backbone

    Use

    Tags

    AID

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAC90-pmax-DEST

    Plasmid

    #48222

    Purpose

    Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR; Gateway destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETNb-nALFA

    Plasmid

    #159988

    Purpose

    For the protein expression with ALFA tag at N-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti-sgRNA-Lib

    Plasmid

    #53121

    Purpose

    sgRNA expression construction in a 3rd generation lentiviral backbone

    Depositor

    Wensheng Wei

    Article

    Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Cmv d0 dPspCas13b-GGS-NYTHDF1

    Plasmid

    #119855

    Purpose

    Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-NYTHDF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP NFAT5 DBD

    Plasmid

    #14112

    Depositor

    Anjana Rao

    Article

    Jauliac et al Nat Cell Biol. 2002 Jul . 4(7):540-4.

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    DNA binding domain aa 175 to 471

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

36. AL13Rb-35S

    Plasmid

    #161008

    Purpose

    PP7-tagged reporter driven by the 35S promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. BirA-Myc_N_SP1

    Plasmid

    #167726

    Purpose

    Expresses SP1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    SP1 (SP1 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MAP72_MS2

    Plasmid

    #220623

    Purpose

    Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBiex-1 Myosin VI HMM_eGFP

    Plasmid

    #82714

    Purpose

    Expresses Myosin VI HMM with a C-terminal eGFP.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Myosin VI
    

    Use

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKDR-TetOn

    Plasmid

    #183577

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated Tet-On promoter)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Unattenuated
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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