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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,163 results for: GRN

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Showing: 10701 - 10750 of 15163 results

  2. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  3. pSDMA67

    Plasmid
    #128356
    Purpose
    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.
    Depositor
    James Fraser
    Article
    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do
    Insert
    Noureothricin (NAT)
    Use
    Unspecified
    Promoter
    ACT1
    Available Since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    128356-plasmid-map-sequence-id-254064

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  4. MTK234_055

    Plasmid
    #123920
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123920-plasmid-map-sequence-id-250070

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  5. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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  13. pCASCADE

    Plasmid
    #65821
    Purpose
    Control guide RNA plasmid E. coli , low phosphate induction
    Depositor
    Michael Lynch
    Insert
    control guideRNA
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Available Since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65821-plasmid-map-sequence-id-111971

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  15. pYPQAT01

    Plasmid
    #223373
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223373-plasmid-map-sequence-id-454171

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  16. pYPQAT04

    Plasmid
    #223376
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223376-plasmid-map-sequence-id-453299

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  25. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Expression
    Bacterial
    Available Since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

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  33. pMOD_C2516b

    Plasmid
    #91085
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91085-plasmid-map-sequence-id-182050

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  40. pYPQAT11

    Plasmid
    #223383
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpRY-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223383-plasmid-map-sequence-id-479119

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  41. pYPQAT08

    Plasmid
    #223380
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpRY-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223380-plasmid-map-sequence-id-452338

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  42. pBHM2651

    Plasmid
    #229993
    Purpose
    Carries AID* tag, empty sgRNA, and NEOR marker
    Depositor
    Hiten Madhani
    Insert
    AID* - empty sgRNA - NEOR
    Tags
    2xFLAG
    Expression
    Bacterial and Yeast
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229993-plasmid-map-sequence-id-461132

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  48. cTRE-GFP-miR30_shPten.1523

    Plasmid
    #135667
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    shPten
    Use
    Mouse Targeting
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135667-plasmid-map-sequence-id-325441

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Showing: 10701 - 10750 of 15163 results