We narrowed to 13,164 results for: cdna

1. pcDNA-RanWT-mRFP1-polyA

   Plasmid

   #59750

   Purpose

   Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran-mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO HIS DNAJB6a

   Plasmid

   #19556

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DNAJB6a (DNAJB6 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-GAlphai3-RLuc8

   Plasmid

   #140975

   Purpose

   Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai3-RLuc8 (GNAI3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA-PHLPP1 N-terminal truncation

   Plasmid

   #22404

   Purpose

   Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

   Depositor

   Alexandra Newton

   Article

   Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

   Insert

   PHLPP1 (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-GFP-Flag-UTR

   Plasmid

   #183235

   Purpose

   To express FLAG tagged eGFP

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   EGFP
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-MTOR-V2006I

   Plasmid

   #69012

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   V2006I

   Promoter

   CMV

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+(rInsp)-Ins-GLuc

   Plasmid

   #89928

   Purpose

   Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

   Depositor

   Melanie Cobb

   Article

   Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

   Insert

   human insulin-Gaussia-Luciferase (INS Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DNA sequence for Gaussia luciferase was humanized…

   Promoter

   rat Insulin II promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

   Plasmid

   #159455

   Purpose

   Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

   Depositor

   Oliver Griesbeck

   Article

   Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

   Insert

   mCRISPRed
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag MKK7B2Jnk1a1(APF)

   Plasmid

   #19730

   Depositor

   Roger Davis

   Article

   Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

   Insert

   MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Human, Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   replaced dual phosporylation motif Thr(1959)-Pro-…

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. GeNL(Ca2+)_520/pcDNA3

    Plasmid

    #85204

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_520
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1478 pcDNA3 flag HA 14-3-3 beta

    Plasmid

    #8999

    Depositor

    William Sellers

    Insert

    14-3-3 beta (YWHAB Human)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1 SARS-CoV-2 S D614

    Plasmid

    #158074

    Purpose

    Expresses SARS-CoV-2 spike protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Bruce Spiegelman, Jorge Ruas

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-SEP-Glur2 (MCA10)

    Plasmid

    #64941

    Purpose

    mammalian expression of rat GluR2

    Depositor

    Jeremy Henley

    Article

    Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

    Insert

    GluR2 (Gria2 Rat)
    

    Use

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA-3xFLAG-NLS-TPP1

    Plasmid

    #53585

    Purpose

    Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Silent mutation to eliminate PacI site

    Promoter

    CMV

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1_3xFlagEzh2 RNA binding mutant

    Plasmid

    #173718

    Purpose

    RNA-binding mutant EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 RNA-binding mutant
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Hygro(+)-PSTCD-cGAS

    Plasmid

    #102605

    Purpose

    Mammalian expression of cGAS fused to PSTCD tag

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)/AUG-nLuc-3XFLAG

    Plasmid

    #127299

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 CHICA (Nigg HS342)

    Plasmid

    #41153

    Depositor

    Erich Nigg

    Article

    Santamaria et al Curr Biol. 2008 May 20;18(10):723-9.

    Insert

    CHICA (FAM83D Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    V11L

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5/FRT/TO_b4g-HaloTag9

    Plasmid

    #175546

    Purpose

    Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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