We narrowed to 19,585 results for: Met;

Showing: 4301 - 4320 of 19585 results

Results per page:

Sort results by:

pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84261

Purpose

Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
PYL1-VPR-p2a-GID1-ABI

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

Plasmid

#35510

Purpose

AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

Plasmid

#35512

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV2

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-eGFP-attP(bxb)-*BsdR

Plasmid

#183751

Purpose

Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

Plasmid

#89873

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

R173H in TdTomato (Please see depositor comments …

Promoter

Human synapsin

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAPDH::H2B-mScarlet

Plasmid

#186764

Purpose

A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

H2B-mScarlet

Use

Overexpression

Tags

H2B

Promoter

GAPDH

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

Plasmid

#104563

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1 (D832A)

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBR_attB(bxb)_ccdB_lox

Plasmid

#183763

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

ccdB

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgp53/Cre

Plasmid

#89646

Purpose

Expresses a p53-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting p53 (Trp53 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mOrange2-Fibrillarin-7

Plasmid

#57957

Purpose

Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Fibrillarin (FBL Human)

Tags

mOrange2

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
Lenti-sgArid1a/Cre

Plasmid

#89642

Purpose

Expresses an Arid1a-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Arid1a (Arid1a Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-REX-GECO1

Plasmid

#61246

Purpose

Expresses REX-GECO1 in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO1

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMis1_1B

Plasmid

#192079

Purpose

A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

Depositor

Markus Buchhaupt

Article

Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

Inserts

KanR
rhaR
rhaS
pBBR1 rep

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Pinducer20-BSD-scFv-sfGFP-DNMT3A1

Plasmid

#115780

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

scFv-sfGFP-DNMT3A1 (DNMT3A Human)

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
DRH335: FCK-Optopatch1

Plasmid

#51695

Purpose

Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch1

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

Plasmid

#89874

Purpose

expresses DRD2-iTango component in mammalian cells for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

DRD2-V2tail-TevN-BLITz1-TetR-VP16

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

short isoform of DRD2 (deletion of 242-270)

Promoter

Human synapsin

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRb1/Cre

Plasmid

#89647

Purpose

Expresses an Rb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Rb1 (Rb1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
5XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111545

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChrimson_tdtomato_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-sCre

Plasmid

#55635

Purpose

Expresses sCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge