We narrowed to 85,550 results for: Son

1. pRK5-SNAP-CLIP-GB1a

   Plasmid

   #125709

   Purpose

   Eukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor

   Depositor

   Kai Johnsson

   Article

   Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

   Insert

   SNAP-CLIP-GB1a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. sfTq2-N1

   Plasmid

   #117931

   Purpose

   Superfolder mTurquoise2 (pNM112)

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   sfTq2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMU-AUTr

   Plasmid

   #61204

   Purpose

   Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   LC3(ATG8)
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET19b+H2B

   Plasmid

   #157809

   Purpose

   Expression of human histone H2B

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H2B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLEX_307_ms2_VPR

   Plasmid

   #79376

   Purpose

   Recruits VPR to compatible gRNAs for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   ms2-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cenp-B INCENP GFP

   Plasmid

   #45238

   Purpose

   Target INCENP to centromeres via full length CENP-B

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B, INCENP-Δcen, GFP
   

   Use

   Tags

   GFP and full length CENP-B

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-shRNA-Tet1

   Plasmid

   #85742

   Purpose

   shRNA against mouse Tet1

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   shRNA Tet1
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 4 gRNA concatemer

   Plasmid

   #84881

   Purpose

   Empty concatmer vector in which 4 gRNAs can be inserted

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLH-sgRNA1-boxB-MS2-PP7

   Plasmid

   #75397

   Purpose

   sgRNA1-boxB-MS2-PP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-boxB-MS2-PP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Sox17 GFP

    Plasmid

    #23334

    Depositor

    Sean Morrison

    Article

    Kim et al Cell. 2007 Aug 10. 130(3):470-83.

    Insert

    Sex determining region Y-box containing gene 17 (Sox17 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR Clover-VISH2

    Plasmid

    #188629

    Purpose

    Fluorescent reporter for SLP76/VISH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    VISH2
    

    Use

    Lentiviral

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-FLAG-Sestrin2-L261A

    Plasmid

    #73671

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L261A changed Leucine 261 to Alanine

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pLEX_307_ms2_p65_hsf1

    Plasmid

    #79374

    Purpose

    Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    ms2-p65-hsf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SAFB1-pcDNA3.1

    Plasmid

    #32742

    Depositor

    Steffi Oesterreich

    Article

    Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

    Insert

    SAFB1 (SAFB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pDB038a

    Plasmid

    #99211

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 29 and 184 of gIII

    Promoter

    Ppsp and PproK

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p5E-QUASM1

    Plasmid

    #155114

    Purpose

    5' entry vector containing QUASM1 promoter element.

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1
    

    Use

    Gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    QUASM1-E1b

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGagMBPHis

    Plasmid

    #42176

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    Gag protein sequence is from HIV-1 clone NL4-3
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pJ211_WT_low

    Plasmid

    #128848

    Purpose

    Low copy number plasmid for expression of E. coli flagellin from its natural promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEO100c

    Plasmid

    #50550

    Purpose

    Expresses sfGFP under the PompC1157 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pDB038b

    Plasmid

    #99212

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 29, 83, and 184 of gIII

    Promoter

    Ppsp and PproK

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSETB-de4GFP

    Plasmid

    #85472

    Purpose

    Inducible expression of dual emitting pH sensitive GFP in bacterial cells

    Depositor

    S. James Remington

    Article

    Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

    Insert

    de4GFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S65T/C48S/H148C/T203C

    Promoter

    T7

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-N1-ro2GFP

    Plasmid

    #82370

    Purpose

    Expression of redox sensitive GFP in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAMP-CY1_NF1 T2 (+minintr)

    Plasmid

    #119205

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 2 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LaM8-AK74

    Plasmid

    #159594

    Purpose

    Opto-Nanobody

    Depositor

    Jared Toettcher, Jose Avalos

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LacI INCENP

    Plasmid

    #45236

    Purpose

    Target INCENP to lac operator sequence

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    LacI, INCENP-Δcen, mCherry (lacI )
    

    Use

    Tags

    LacI (lac repressor protein) and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    t7

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDB070.iodo.5

    Plasmid

    #99397

    Purpose

    Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    PN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PN25, Ptet, and PproK

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 2 gRNA concatmer

    Plasmid

    #84879

    Purpose

    Empty concatmer vector in which 2 gRNAs can be inserted

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

28. pDB007ns2a

    Plasmid

    #99214

    Purpose

    Accessory plasmid for PACE of PylRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    

    Use

    Tags

    Expression

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and Ptet

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA6-HA-AML1-ETO

    Plasmid

    #12508

    Depositor

    Dong-Er Zhang

    Article

    Peterson et al Blood. 2007 May 15;109(10):4392-8. Epub

    Insert

    HA-AML1-ETO (RUNX1T1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

30. YFP-Rac2(V12)

    Plasmid

    #11397

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Glycine 12 changed to Valine.

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRK5-FLAG-Sestrin2-E451A

    Plasmid

    #73673

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pAMP-CY1_NF1 T1 (+minintr, KDR)

    Plasmid

    #119200

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 1 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. DGK alpha

    Plasmid

    #35404

    Depositor

    Stephen Prescott, Robert Lefkowitz

    Article

    Nelson et al Science. 2007 Feb 2;315(5812):663-6.

    Insert

    DGK alpha (DGKA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pME-Fv2E-flt4um200-ns

    Plasmid

    #67696

    Purpose

    middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    Fv2E-flt4um200
    

    Use

    Tags

    HA

    Expression

    Mutation

    um200, no stop codon; N1056S

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p5E-mcs2

    Plasmid

    #138362

    Purpose

    Gateway compatible 5' entry clone contains mutli-cloning sites sequence2.

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    sequence multi cloning sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMiniT:SEP-beta2 TKIT donor

    Plasmid

    #169436

    Purpose

    Donor DNA for TKIT knockin of SEP to Gabrb2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SiT-EGFP

    Plasmid

    #166943

    Purpose

    mammalian expression of N' Sialyl Transferase tagged with EGFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Cell. 2008 Jun 13;133(6):1055-67. doi: 1

    Insert

    Sialyl Transferase N-terminus
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRSETB-ro2GFP

    Plasmid

    #82366

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/S65T/Q80R/S147C/Q204C

    Promoter

    T7

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDB023f1

    Plasmid

    #99218

    Purpose

    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD4] T7RNAP(S12*,S203*)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid

    #67279

    Purpose

    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    

    Use

    Tags

    linked to FlpO through an P2A ribosomal skipping …

    Expression

    Mammalian

    Mutation

    Promoter

    pdx

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge