Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

164,909 results

Showing: 10751 - 10800 of 164909 results

  6. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Article
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    113865-plasmid-map-sequence-id-220242

    Enlarge

  8. ORFtag_TetR-SD_Frame1

    Plasmid
    #220981
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220981-plasmid-map-sequence-id-437540

    Enlarge

  16. CIDAR MoClo Extension, Volume I

    Plasmid Kit
    #1000000161
    Purpose
    This kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.
    Depositor
    Richard Murray
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. pLV-REPORT (PGK) NanoLuc

    Plasmid
    #208576
    Purpose
    Empty NanoLuc Lentiviral reporter
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    NanoLuciferase
    d2eGFP
    Empty response element
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    3x SV40 NLS and PEST
    Expression
    Mammalian
    Mutation
    Promoter
    PGK and miniP
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    208576-plasmid-map-sequence-id-413002

    Enlarge

  20. SPLINTR mCHERRY v1

    Pooled Library
    #179775
    Purpose
    SPLINTR (Single-cell Profiling and LINeage TRacing) is a high diversity lentiviral barcode library for tracing clonal lineages using bulk and single-cell readouts.
    Depositor
    Mark Dawson
    Article
    Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:
    Expression
    Mammalian
    Use
    Lentiviral
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only

  24. ADRB2-Tango

    Plasmid
    #66220
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66220-plasmid-map-sequence-id-247680

    Enlarge

  30. pBad-CA

    Plasmid
    #105665
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105665-plasmid-map-sequence-id-205511

    Enlarge

  32. PB-UBC-GCAMP6f-PGK-neo

    Plasmid
    #160049
    Purpose
    expression of GCaMP6f in a piggybac plasmid
    Depositor
    Xiaojun Lian
    Insert
    GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ubc
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    160049-plasmid-map-sequence-id-313848

    Enlarge

  36. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

    Enlarge

  39. pAAV-Ef1a-DIO ChETA-EYFP (AAV5)

    Viral Prep
    #26968-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  42. NL4-3 mCherry Luciferase

    Plasmid
    #44965
    Purpose
    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
    Depositor
    Warner Greene
    Article
    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371
    Insert
    mCherry, Luciferase, IRES
    Use
    Lentiviral
    Tags
    Luciferase, IRES and mCherry with D212G
    Expression
    Mutation
    S34C mutation in Nef
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    44965-plasmid-map-sequence-id-352462

    Enlarge

  45. AAV-CamKIIa-jGCaMP8f-WPRE

    Plasmid
    #176750
    Purpose
    AAV-mediated expression of GCaMP8f under the CamKIIa promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176750-plasmid-map-sequence-id-408507

    Enlarge

  48. pAAV-Ef1a-DIO-PPO-Venus (AAV8)

    Viral Prep
    #139505-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Promoter
    EF1a
    Tags
    Venus (Cre-dependent)
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  50. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid
    #108244
    Purpose
    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression
    Depositor
    Jerome Bonnet
    Article
    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102
    Insert
    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    pLacO1
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108244-plasmid-map-sequence-id-210116

    Enlarge

Showing: 10751 - 10800 of 164909 results