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171,853 results

Showing: 10751 - 10800 of 171853 results

  8. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

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  11. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid
    #105537
    Purpose
    AAV expression of Cre from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105537-plasmid-map-sequence-id-202428

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  13. AiP14801 - pAAV-AiE0390m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4801)

    Plasmid
    #208163
    Purpose
    AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    iCreR297T
    Use
    AAV
    Mutation
    NA
    Promoter
    Beta Globin minimal promoter
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208163-plasmid-map-sequence-id-429391

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  15. hCD12

    Plasmid
    #225541
    Purpose
    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    225541-plasmid-map-sequence-id-446604

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  20. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  24. pD649-HAsp-CD22-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156940
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD22 (CD22 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156940-plasmid-map-sequence-id-401025

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  27. mTurquoise-Rab5a-7

    Plasmid
    #55578
    Purpose
    Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Rab5a
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. mCherry-ATG5-C-18

    Plasmid
    #54995
    Purpose
    Localization: Membrane Receptor, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ATG5 (ATG5 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. mEmerald-Integrin-Beta1-N-18

    Plasmid
    #54129
    Purpose
    Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Integrin-Beta1 (ITGB1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54129-plasmid-map-sequence-id-314085

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  48. pcDNA_mIGg2a_Anti-HA [12CA5] v2

    Plasmid
    #236295
    Purpose
    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse IgG2a heavy chain; to be used with pcDNA_mK_Anti-HA [12CA5] v2 light chain (Plasmid 236296) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] heavy chain variable region fused to mouse IgG2a heavy chain
    Tags
    Mouse IgG2a Fc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236295-plasmid-map-sequence-id-467832

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  49. pcDNA_mK_Anti-HA [12CA5] v2

    Plasmid
    #236296
    Purpose
    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse Kappa light chain; to be used with pcDNA_mIGg2a_Anti-HA [12CA5] v2 heavy chain (Plasmid 236295) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] light chain variable region fused to mouse kappa light chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236296-plasmid-map-sequence-id-467834

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Showing: 10751 - 10800 of 171853 results