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173,478 results

Showing: 10751 - 10800 of 173478 results

  2. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  5. pMAL-c5x_UnaG

    Plasmid
    #163125
    Purpose
    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.
    Depositor
    Julie Biteen
    Article
    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi
    Insert
    UnaG
    Tags
    His and MBP
    Expression
    Bacterial
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163125-plasmid-map-sequence-id-324911

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  7. pALS3-Ma-sfGFP-WT

    Plasmid
    #212119
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212119-plasmid-map-sequence-id-444185

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  8. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Article
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Tags
    8*His and GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85822-plasmid-map-sequence-id-183287

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  10. pET-MCN-10His-mStayGold (E138D)

    Plasmid
    #211361
    Purpose
    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Article
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Insert
    mStayGold
    Tags
    10xHis-tag
    Expression
    Bacterial
    Mutation
    E138D
    Promoter
    T7
    Available Since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    211361-plasmid-map-sequence-id-417678

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  14. pUltra-Plus-H2B-mTagBFP2

    Plasmid
    #161790
    Purpose
    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Bicistronic
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC and UbC promoter
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161790-plasmid-map-sequence-id-316820

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  15. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid
    #155189
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP
    Depositor
    Xiaoping Bao
    Article
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Insert
    beta-catenin (Ctnnb1 Mouse)
    Use
    Unspecified; Piggybac transposon
    Tags
    P2A eGFP NLS
    Expression
    Mammalian
    Mutation
    Sites phosphorylated by GSK-3beta have been mutat…
    Promoter
    TRE3G
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155189-plasmid-map-sequence-id-307963

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  16. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

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  21. pNJP

    Plasmid
    #115494
    Purpose
    Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d
    Insert
    NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Synthetic, Human, Mouse)
    Tags
    iRFP, mCherry, and mKO
    Expression
    Mammalian
    Mutation
    mCherry S227F, mCherry G229R, mKO V211G
    Promoter
    CAG
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115494-plasmid-map-sequence-id-231521

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  28. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  43. eIF2a 2

    Plasmid
    #21808
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Mutation
    S52A
    Available Since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21808-plasmid-map-sequence-id-229779

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Showing: 10751 - 10800 of 173478 results