We narrowed to 20,059 results for: ato

1. pFX-mito-MiCy

   Plasmid

   #174177

   Purpose

   Express MiCy with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   MiCy with mitochondria-targeting sequence
   

   Use

   Tags

   the mitochondria-targeting sequence of the cytoch…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMuLE ENTR CMV L1-R5

   Plasmid

   #62090

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFX-Lifeact-mCherry

   Plasmid

   #175822

   Purpose

   Express mCherry with actin-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with actin-targeting sequence
   

   Use

   Tags

   Lifeact (N-terminal 17 amino acids from S. Cerevi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMB

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMuLE ENTR SV40 L5-L2

   Plasmid

   #62095

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1+N-eGFP_SAMD9L_R986C

   Plasmid

   #206176

   Purpose

   mammalian expression of SAMD9L R986C

   Depositor

   Christopher Goodnow

   Article

   Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

   Insert

   SAMD9L (SAMD9L Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R986C

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMP-G9A_3

   Plasmid

   #36333

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   G9A (EHMT2 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pJet-CMV-CD5Flag2-bglpA

   Plasmid

   #89705

   Purpose

   Expression plasmid for Flag embedded into human CD52 with short polyA

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CMV-CD5Flag2-bglpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DsRed2_pLENTI-CAG-IRES-GFP

   Plasmid

   #177010

   Purpose

   Mammalian lentiviral expression vector encoding DsRed2

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   DsRed2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-PIWI

   Plasmid

   #11591

   Depositor

   Edward Chan

   Article

   Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

   Insert

   Ago2 (AGO2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain only aa482-869

   Promoter

   Available since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pGEX Smad2 (L+MH2)

    Plasmid

    #15730

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    Smad2 (L+MH2) (SMAD2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 187-467 (linker and MH2 regions).

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pFA6a-His3MX6-PGAL1-3HA

    Plasmid

    #41610

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAd FLAG HDAC5 WT

    Plasmid

    #32209

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pGP-CMV-NES-fRGECO1

    Plasmid

    #125247

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLN472 (FuGW-G8p-STE-1Pe)

    Plasmid

    #105190

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of STE transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-STE-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KHBD00233

    Plasmid

    #34377

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7803 (z Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. MTK0_015

    Plasmid

    #123930

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - Insulator + tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CoV-NSP3-c001

    Plasmid

    #173082

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1000-K1173, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMuLE ENTR MCS L5-L4

    Plasmid

    #62089

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMuLE ENTR mCherry L5-L2

    Plasmid

    #62184

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing mCherry. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    mCherry
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFX-β-tubulin-iRFP

    Plasmid

    #175830

    Purpose

    Express iRFP with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with microtuble-targeting sequence
    

    Use

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGREAT18

    Plasmid

    #170906

    Purpose

    GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pAtUBQ10

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p3×FLAG-CMV-14-LanCL2

    Plasmid

    #73391

    Purpose

    Expresses FLAG tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Use

    Tags

    3x FLAG tagged

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-CMV-CIBN-mCherry-VAMP2

    Plasmid

    #178573

    Purpose

    Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CIBN-mCherry-VAMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lentiCRISPR-v2-Blast-Puro

    Plasmid

    #167186

    Purpose

    Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMuLE ENTR eGFP L5-L2

    Plasmid

    #62183

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing EGFP. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    eGFP
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQsupR-Mi2b

    Plasmid

    #15379

    Depositor

    Stephen Smale

    Article

    Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

    Insert

    short hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pBad-CA TAG126

    Plasmid

    #105836

    Purpose

    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 126

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIVT/Smarcc1

    Plasmid

    #122145

    Purpose

    For making Smarcc1 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarcc1 (Smarcc1 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGBT-R16

    Plasmid

    #31827

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    mini Tol2 ITR
    

    Use

    Cre/Lox; Gene trap

    Tags

    mRFP

    Expression

    Mutation

    mRFP lacks start codon

    Promoter

    beta Actin

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

    Plasmid

    #84258

    Purpose

    Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgTRE3G
    PYL1-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pL0_EF-H4

    Plasmid

    #202178

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    H4 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pL0_EF-fcpA

    Plasmid

    #202164

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    fcpA terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pSMP-MBD3_3

    Plasmid

    #36373

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD3 (MBD3 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pCMV-dMASS_1

    Plasmid

    #209764

    Purpose

    Expression of dMASS_1 in mammalian cells under CMV promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBad-CA TAG20

    Plasmid

    #105666

    Purpose

    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 20

    Promoter

    pBad/arabinose

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMuLE ENTR MCS L1-R5

    Plasmid

    #62084

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MG15-gal (mut p53 binding sites)

    Plasmid

    #16447

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    p53-binding site (mutant) (TP53 )
    

    Use

    Beta-gal reporter

    Tags

    beta-gal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

38. pUAST-Myc-dATG1

    Plasmid

    #111801

    Purpose

    Insect Expression of dAtg1

    Depositor

    Jun Hee Lee

    Article

    Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

    Insert

    dAtg1 (Atg1 Fly)
    

    Use

    Tags

    Myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CamKII-DIO-hM4D(Gi)-mCherry

    Plasmid

    #190240

    Purpose

    Expresses iDREADD fused to mCherry flanked by DIO

    Depositor

    Young J Yoon

    Article

    Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    mouse CamKII alpha

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

    Plasmid

    #129704

    Purpose

    Express HA-KASH for nuclei labeling and purification

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    HA-VHH-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCFL-mPML-3KRmut-iB

    Plasmid

    #140287

    Purpose

    Expression of mouse PML KR mutant

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    PML (Pml Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.2/2K-NS4B

    Plasmid

    #120827

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    2K-NS4B ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET-GST-TNRC6A-(935-984)

    Plasmid

    #42050

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    contain aa935-984

    Promoter

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMuLE ENTR U6-miR-30 L1-R5

    Plasmid

    #62113

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. spa-GCaMP6s

    Plasmid

    #67556

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pFX-Venus-Sec61β

    Plasmid

    #174467

    Purpose

    Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pfastbac/mBmal62-447

    Plasmid

    #47335

    Purpose

    mBmal fragment cloned into pfastbac and crystallized

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    aa 62-447

    Promoter

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

48. Lenti-sgKmt2d-sg3

    Plasmid

    #192167

    Purpose

    Lenti-sgKmt2d-sg3

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3.2/NS4A-2K

    Plasmid

    #120820

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS4A + 2K ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA-NES-ZapCY2

    Plasmid

    #59016

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

    Insert

    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only