We narrowed to 6,886 results for: GFP expression plasmids

1. int>pro::GFP

   Plasmid

   #218576

   Purpose

   Transcriptional reporter for ARF7 promoter

   Depositor

   Anthony Bishopp

   Insert

   AT5G20730 (NPH4 Mustard Weed)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Insert genomic sequence 41-372nt to -46nt upstrea…

   Promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. int>UTR::GFP

   Plasmid

   #218577

   Purpose

   Transcriptional reporter for ARF7 promoter

   Depositor

   Anthony Bishopp

   Insert

   AT5G20730 (NPH4 Mustard Weed)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Insert genomic sequence 41-372nt to -622nt upstre…

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPaint-T2A-sfGFP-3xP3-RFP

   Plasmid

   #127557

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-C-moxGFP-Blast-H2BC11

   Plasmid

   #207757

   Purpose

   Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. qTAG-C-moxGFP-Puro-H2BC11

   Plasmid

   #207758

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. qTAG-C-moxGFP-Zeo-H2BC11

   Plasmid

   #207759

   Purpose

   Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. PM-GFP

   Plasmid

   #21213

   Depositor

   Tobias Meyer

   Article

   Teruel et al J Neurosci Methods. 1999 Oct 30. 93(1):3

   Insert

   PM-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PM targetting sequence is Lyn (MGCIKSKRKD) (Lab …

   Promoter

   Available since

   July 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. U371-GFP

   Plasmid

   #24476

   Depositor

   Ron Vale

   Article

   Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

   Insert

   Unc104 (unc-104 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Contains only the kinesin's motor head fused…

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pSAH46 - MosTI [unc-119(partial) | MCS | gfp]

   Plasmid

   #200366

   Purpose

   Cloning vector for mosTI(unc-119, gfp). MCS or Golden Gate.

   Depositor

   Christian Froekjaer-Jensen

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid

    #140030

    Purpose

    Tunable and temporal expression control of NFIL3 and nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    NFIL3 (NFIL3 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

    Plasmid

    #199781

    Purpose

    Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

    Depositor

    Sascha Martens

    Insert

    NEMO (IKBKG Human)
    

    Use

    Tags

    GST-Thrombin-TEV-EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-PGK-eGFP

    Plasmid

    #193023

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (PGK) promoter

    Depositor

    Bart Nieuwenhuis

    Article

    Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Anti-GFP [N86/34R]

    Plasmid

    #220419

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Anti-GFP [N86/38R-1]

    Plasmid

    #206719

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/38-1.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant Mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Anti-GFP [N86/20R]

    Plasmid

    #188166

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/20

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Anti-GFP [N86/44R]

    Plasmid

    #188164

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/44

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGFP-LacI

    Plasmid

    #179510

    Purpose

    Expresses EGFP-LacI in mammalian cells

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LacI
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NSP3C-EGFP

    Plasmid

    #210318

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-EGFP-C1-HsNot1iso1_1085-1605-del1_V

    Plasmid

    #147750

    Purpose

    Mammalian Expression of HsNot1iso1_1085-1605-del1

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso1_1085-1605-del1 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1 silent mutation compared to the sequence of iso…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-EGFP-C1-HsNot1iso1_1085-1605-del2_V

    Plasmid

    #147751

    Purpose

    Mammalian Expression of HsNot1iso1_1085-1605-del2

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso1_1085-1605-del2 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1 silent mutation compared to the sequence of iso…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT7-EGFP-C1-HsNot1iso1_1085-1605-del3_V

    Plasmid

    #147752

    Purpose

    Mammalian Expression of HsNot1iso1_1085-1605-del3

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso1_1085-1605-del3 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1 silent mutation compared to the sequence of iso…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. integrin alpha9 / alpha4 EGFP-N3

    Plasmid

    #13602

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 / alpha 4 (ITGA9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. integrin alpha9 / alpha2 EGFP-N3

    Plasmid

    #13601

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 / alpha 2 (ITGA9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. integrin alpha9 / alpha5 EGFP-N3

    Plasmid

    #13603

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 / alpha 5 (ITGA9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDEST47-ARL5A-GFP

    Plasmid

    #67399

    Purpose

    Mammalian expression of hArl5 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL5A (ARL5A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    bp 70 C to T; silent mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

26. CAG-loxP-3xpA-sfGFP-tdTomato T2A V XE

    Plasmid

    #113853

    Purpose

    Superfolder GFP and tdTomato linked by T2A (V linker)

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-0.5Syn-Exon 1 of BNDF-GFP-WPRE

    Plasmid

    #233059

    Purpose

    To express GFP from a 0.5 bp synapsin promoter. The non-coding BDNF exon one is 5' to the BDNF start codon.

    Depositor

    Jonathan Ploski

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP

    Plasmid

    #197370

    Purpose

    The lentiviral vector for miRFP670nano(R57C/C86S) and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    miRFP670nano(R57C/C86S)
    

    Use

    Lentiviral

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    R57C/C86S mutation

    Promoter

    CAG

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP

    Plasmid

    #198062

    Purpose

    The lentiviral vector for iRFP713(C15S/V256C) and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP713(C15S/V256C)
    

    Use

    Lentiviral

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    C15S/V256C mutation

    Promoter

    CAG

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CAG-loxP-3xpA-sfGFP-tdTomato T2A Q XE

    Plasmid

    #113852

    Purpose

    Superfolder GFP and tdTomato linked by T2A (Q linker)

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid

    #121169

    Purpose

    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    FNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

    Plasmid

    #177182

    Purpose

    plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    pegRNA targeting the PEAR-GFP plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-anti-GFP(LaG2) PAGER(TF)

    Plasmid

    #229996

    Purpose

    Expresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

    Plasmid

    #113862

    Purpose

    Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-NLuc1.1
    12GLI-Fluc
    CBF-GLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-anti-GFP(LaG17) PAGER(TF)

    Plasmid

    #229997

    Purpose

    Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FLT3-D835Y-MSCV-IRES-GFP

    Plasmid

    #184780

    Purpose

    Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Activating tyrosine kinase domain mutation (D835Y)

    Promoter

    MSCV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

    Plasmid

    #110376

    Purpose

    Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pmGFP11C-­Tau

    Plasmid

    #71434

    Purpose

    Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

    Depositor

    Henri Huttunen

    Article

    Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

    Insert

    Tau (0N4R) (MAPT Human)
    

    Use

    Tags

    GFP (213-228)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

41. pmGFP10C-­Tau

    Plasmid

    #71433

    Purpose

    Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

    Depositor

    Henri Huttunen

    Article

    Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

    Insert

    Tau (0N4R) (MAPT Human)
    

    Use

    Tags

    GFP (1-212)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

42. pDR-GFPuniv

    Plasmid

    #46085

    Purpose

    In vivo recombination assay fluorescent reporter

    Depositor

    Raymond Monnat

    Article

    Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

    Insert

    DR-GFPuniv reporter
    

    Use

    Recombination reporter plasmid

    Tags

    Expression

    Mammalian

    Mutation

    E5G and K163R mutations in EGFP relative to wild-…

    Promoter

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. p4B5-EGFP

    Plasmid

    #140324

    Purpose

    Detection of Dengue virus infection in live cells

    Depositor

    Alan Rothman

    Article

    Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

    Insert

    DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    2 conservative n.t. differences near 5' end …

    Promoter

    CMV

    Available since

    May 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid

    #212120

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    

    Use

    Tags

    His6 and SUMO-TAG35

    Expression

    Bacterial

    Mutation

    E35TAG (within SUMO encoding sequence)

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. Venus-Bik-cb5-pEGFP-C1

    Plasmid

    #177431

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Bik MBR removed (after position 136) and replaced…

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. groSL-GFP

    Plasmid

    #109389

    Purpose

    GFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pgroSL-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. ibpAB-GFP

    Plasmid

    #109390

    Purpose

    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pibpAB-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. htpG1-GFP

    Plasmid

    #109387

    Purpose

    GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    phtpG1-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. htpG2-GFP

    Plasmid

    #109388

    Purpose

    GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    phtpG2-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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