We narrowed to 2,366 results for: dCas9

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLX303-ZIM3-KRAB-dCas9

Plasmid

#154472

Purpose

Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Mutation

D10A and H840A

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-KRAB-MeCP2

Plasmid

#122267

Purpose

SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Tags

dCas9-KRAB-MeCP2

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-p300 Core

Plasmid

#61357

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pIND20-KNL1-rvsf_aaaa-dCas9

Plasmid

#248562

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

TRE, miniCMV

Available Since

May 15, 2026

Availability

Academic Institutions and Nonprofits only

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Article

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-DD-KNL1-rvsf_aaaa-dCas9

Plasmid

#248565

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

DD-KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pIVT_superDn29-dCas9-P2A-GFP

Plasmid

#247166

Purpose

In vitro transcription of human codon optimized fusion of superDn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Article

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE TRE dCas9-KRAB

Plasmid

#50917

Purpose

Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

TRE

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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dCas9-p300 PHD-HAT

Plasmid

#179545

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-KNL1-rvsf_aaaa-dCas9

Plasmid

#248567

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-IBM1 (D30)

Plasmid

#239492

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-Tet1v4-dCas9

Plasmid

#232180

Purpose

Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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