We narrowed to 27,344 results for: nes

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pDF0352 HvsCas7-11 with C-term Nes mammalian expression

Plasmid

#172516

Purpose

Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

C-term Nes HvsCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173875

Purpose

Mammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax

Plasmid

#173876

Purpose

Mammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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nes374tk/lacZ

Plasmid

#47615

Purpose

used to create transgenic mice expressing LacZ reporter with Nestin enhancer

Depositor

Urban Lendahl

Article

Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.

Insert

nestin 2nd intron fragment (NES Human)

Use

Enhancer reporter

Tags

HSV tk promoter and lacZ

Mutation

374 bp from 2nd intron (comprising bp 1153 to 152…

Promoter

HSV tk promoter

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173872

Purpose

Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-SomaFRCaMPi

Plasmid

#232837

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted FRCaMPi

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Has Service

AAV8

Insert

NES-SomaFRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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PB-NES-ZapCV2

Plasmid

#222950

Purpose

Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

Depositor

Amy Palmer

Article

Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

Insert

NES-ZapCV2

Tags

ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

Expression

Mammalian

Mutation

ECFP gene is truncated 36 bp at 3' end. Venu…

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NES

Plasmid

#175015

Purpose

Cytosol-targeted TORCAR.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

TORCAR-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDNA3-Cerulean-FHA1-NES

Plasmid

#138202

Purpose

Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

Cerulean-FHA1-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Plasmid

#173871

Purpose

Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-FRCaMPi-P2A-mGreenLantern

Plasmid

#232841

Purpose

AAV transfer plasmid for CAG-mediated co-expression of FRCaMPi and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-FRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-FRCaMPi

Plasmid

#232838

Purpose

AAV transfer plasmid for Syn-mediated expression of FRCaMPi

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomaFRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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piGECI-NES-N1

Plasmid

#160422

Purpose

Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pzk490-pmax-NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

Plasmid

#196843

Purpose

Express NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-syn-NES-jGCaMP8f-WPRE

Plasmid

#186035

Purpose

AAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoter

Depositor

Loren Looger

Insert

NES-jGCaMP8f

Use

AAV

Tags

NES-6xHis

Expression

Mammalian

Promoter

synapsin

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-TagRFP-NES

Plasmid

#184449

Purpose

Cytosolic TagRFP expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

TagRFP-NES

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CytoCKAR

Plasmid

#14870

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Tags

CFP, NES, and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pzk488-pmax-NES-PUFc-FseI[ADAR2-DD-splitN]

Plasmid

#196841

Purpose

Express NES-PUFc-FseI[ADAR2-DD-splitN]

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

NES-PUFc-FseI[ADAR2-DD-splitN]

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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