We narrowed to 16,945 results for: RAN-1
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Plasmid#175746PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 1DepositorInsertmCherry-LOV2-NoLS (HIV Rev transactivating protein)
TagsmCherryExpressionMammalianAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP-INPP5E(D556A)
Plasmid#183678PurposeRecruitable human type IV 5-phosphatase enzyme (catalytically inactive)DepositorInsertINPP5E (INPP5E Human)
TagsmRFP-FKBP12(1-108)ExpressionMammalianMutationC641A, D556APromoterCMVAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-ancestral-retroCHMP3-HA
Plasmid#154248Purposeexpresses ancestral New World monkey retroCHMP3 in mammalian cellsDepositorInsertreconstructed ancestral sequence of New World monkey retroCHMP3
TagsHAExpressionMammalianPromoterEF1-alphaAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-owl-monkey-retroCHMP3-HA
Plasmid#154249Purposeexpresses owl monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
TagsHAExpressionMammalianPromoterEF1-alphaAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-owl-monkey-retroCHMP3(148)-HA
Plasmid#154252Purposeexpresses prematurely truncated owl monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
TagsHAExpressionMammalianMutationC-terminal truncation of retroCHMP3 at amino acid…PromoterEF1-alphaAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP214-Lenti-0.5Synapsin P-GFP- WPRE-pA
Plasmid#62929PurposepLenti vector backbone designed to express GFP from a 0.5Synapsin promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralPromoter0.5synapsin and 1.3αCaMKIIAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRoCascade_gfp
Plasmid#178771PurposeExpression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalisDepositorInsertRocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRoTarget
Plasmid#178774Purposepartial tRNA-Leugene from Rippkaea orientalisDepositorInsertpartial tRNA-Leugene from Rippkaea orientalis
ExpressionBacterialAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PGEX-DDX21-D2+C
Plasmid#179243Purposeprotein purification in E. coliDepositorAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso3_C
Plasmid#146088PurposeMammalian Expression of HsTNRC6Biso3DepositorInsertHsTNRC6Biso3 (TNRC6B Human)
ExpressionMammalianMutationAA 3-188 and AA residues 242-1029 (with N242D) co…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-PBZC-Linker-eGFP
Plasmid#176070PurposeEGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassetteDepositorInsertPBZ-C
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-BRCT2-Linker-eGFP
Plasmid#176085PurposeEGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassetteDepositorAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-BRCT1-Linker-eGFP
Plasmid#176074PurposeEGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassetteDepositorAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-PBM-Linker-eGFP
Plasmid#176069PurposeEGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassetteDepositorInsertPBM
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-KR-Linker-eGFP
Plasmid#176066PurposeEGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassetteDepositorInsertKR
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-FHA-Linker-eGFP
Plasmid#176065PurposeEGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassetteDepositorInsertFHA
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0770
Plasmid#177060PurposeMoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertloganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0121
Plasmid#177053PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoterDepositorInsertiridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0117
Plasmid#177049PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoterDepositorInsertiridoid synthase (CrISY) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0112
Plasmid#177044PurposeMoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertgeraniol synthase (CrGES) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0109
Plasmid#177042PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0108
Plasmid#177041PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoterDepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
COG4 shRNA
Plasmid#163861PurposeshRNA targeting the coding sequences of COG4DepositorInsertcomponent of oligomeric golgi complex 4 (COG4 Human)
ExpressionMammalianAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-K417N
Plasmid#177969PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, K417N)DepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-N439K
Plasmid#177970PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N439K)DepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-L452R
Plasmid#177971PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)DepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-S477N
Plasmid#177972PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)DepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-N501Y
Plasmid#177975PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)DepositorAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2_LPT1
Plasmid#176253PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT geneDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1
Plasmid#176250PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozymDepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux_crRNA NR1
Plasmid#176244PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRhaQCas-BsaI
Plasmid#170631PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
ExpressionBacterialAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) WT
Plasmid#134690PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression and purificationDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) G361C
Plasmid#134694PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
TagsGSTExpressionBacterialMutationG361C, C145A, C217A, C249A, C290A, C338A, C387AAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha (NLS-P426)
Plasmid#171538PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
ExpressionMammalianMutationan SV40 NLS sequence (PKKKRKVGI) insertion into t…Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-rtTAc
Plasmid#172121PurposeConstitutive expression of InteinC-rtTACDepositorInsertInteinC-rtTAc
UseAAVPromoterEF1aAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCaMKII-EBFP-tTAn
Plasmid#172124PurposeExpression of EBFP and tTA-InteinN in CaMKII positive cellsDepositorInsertpCaMKII, EBFP, tTAn-InteinN
UseAAVPromoterEF1aAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-gB-PuroR [M1G]
Plasmid#171803PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-gB-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Zeo-eGFP
Plasmid#162743PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
Plasmid#172195PurposeAAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationtranscription regulatory domain removedPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTM-RBDv2
Plasmid#162785PurposeSARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cellsDepositorInsertSARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
Tags10xHis, c-myc, and hTPA leaderExpressionMammalianPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBCMV-MS2CP-eDHFR
Plasmid#167309PurposeTemplate pDNA to prepare a split CaVT component mRNADepositorInsertMS2CP-eDHFR
UseSynthetic BiologyTagsDYK-tagExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-mgp100-PuroR [M1G]
Plasmid#171802PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-mgp100-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNAP-MIDAS
Plasmid#171604PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
TagsHis6 tag and SNAP tagExpressionBacterialAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG R106L-CPTP
Plasmid#170744PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG K60A-CPTP
Plasmid#170743PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD
Plasmid#168161PurposeInducible expression of PmcCAST TnsAB and TnsC proteins.DepositorInsertPmcCAST Tns proteins (TnsAB and TnsC)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS26: pTns(PmcCAST)_ΔTniQ
Plasmid#168159PurposeInducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.DepositorInsertPmcCAST Tns proteins (TnsAB, TnsC and TnsD)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS27: pTns(PmcCAST)_ΔTnsD
Plasmid#168160PurposeInducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.DepositorInsertsPmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS24: pCascade(PmcCAST)_entry
Plasmid#168157PurposeInducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.DepositorInsertsPmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only