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Showing: 11301 - 11350 of 11732 results

  10. pGEX2T-TEV CHMP1B 4-199 L188A/L192A

    Plasmid
    #108289
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108289-plasmid-map-sequence-id-245274

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  11. pGEX2T-TEV CHMP1B 4-199 L192A/L195A

    Plasmid
    #108291
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 192 to A, changed L 195 to A
    Available Since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108291-plasmid-map-sequence-id-245276

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  17. EGFP-uTEV1-MAVS-mTA3

    Plasmid
    #171001
    Purpose
    HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIV]
    Promoter
    pTRE-tight (TetOn)
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171001-plasmid-map-sequence-id-335916

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  22. pDUAL_2_lacZ

    Plasmid
    #155173
    Purpose
    see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155173-plasmid-map-sequence-id-310557

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  23. pDUAL_araBAD

    Plasmid
    #155172
    Purpose
    BaSP and CuCBP co-expression: araBAD promoter controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    155172-plasmid-map-sequence-id-311670

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  24. pDUAL_2_tacI

    Plasmid
    #155174
    Purpose
    see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155174-plasmid-map-sequence-id-310560

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  25. pDUAL_lacZ

    Plasmid
    #155169
    Purpose
    BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155169-plasmid-map-sequence-id-310554

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  26. pBICI_2_medium

    Plasmid
    #155167
    Purpose
    see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155167-plasmid-map-sequence-id-310552

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  27. pBICI_strong

    Plasmid
    #155165
    Purpose
    BaSP and CuCBP co-expression: strong RBS controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155165-plasmid-map-sequence-id-310539

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  28. pBICI_medium

    Plasmid
    #155164
    Purpose
    BaSP and CuCBP co-expression: medium RBS controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155164-plasmid-map-sequence-id-310538

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  29. pBICI_weak

    Plasmid
    #155163
    Purpose
    BaSP and CuCBP co-expression: weak RBS controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155163-plasmid-map-sequence-id-310535

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  44. FB026

    Plasmid
    #119711
    Purpose
    Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter gpdA:YFP coding sequence:Terminator trpC
    Use
    Synthetic Biology
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119711-plasmid-map-sequence-id-236966

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  45. ORAI1-YFP V107M-L273D-L276D

    Plasmid
    #114183
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M/L273D/L276D
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114183-plasmid-map-sequence-id-218685

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  46. ORAI1-YFP T184M-L273D-L276D

    Plasmid
    #114184
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    T184M/L273D/L276D
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114184-plasmid-map-sequence-id-220961

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  47. pUC19-Q8DTE3

    Plasmid
    #103129
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q8DTE3(Cas9 coding gene from Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103129-plasmid-map-sequence-id-201893

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  48. pUC19-D3UFL8

    Plasmid
    #103095
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    D3UFL8(Cas9 coding gene from Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103095-plasmid-map-sequence-id-201805

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Showing: 11301 - 11350 of 11732 results