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We narrowed to 23,588 results for: CHI

Showing: 11351 - 11400 of 23588 results
  1. pUE1-TSP-ORF68 K450A/R451A

    Plasmid
    #162653
    Purpose
    Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
    Depositor
    Insert
    ORF68
    Use
    Tags
    N-terminal 2xStrep-Tag II and HRV 3C site
    Expression
    Mammalian
    Mutation
    Amino acids K450 and R451 mutated to alanine
    Promoter
    CMV
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid
    #160890
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Insert
    PT7-Nt-rbcS-S1H
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-SH1: T7, chaperones: T13
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pCDF-NtXNtT4aR1AtR2NtB2

    Plasmid
    #160887
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Insert
    PT7-Nt-rbcS-T4a
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-T4a: T7, chaperones: T10
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pDUAL_2_lacZ

    Plasmid
    #155173
    Purpose
    see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. SMT215

    Plasmid
    #134818
    Purpose
    SMT205 with DHFR-FLAG-tag, western blot
    Depositor
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. SMT101

    Plasmid
    #134815
    Purpose
    Dual expression of DHFR and TYMS, in vivo assays
    Depositor
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pDUAL_araBAD

    Plasmid
    #155172
    Purpose
    BaSP and CuCBP co-expression: araBAD promoter controls BaSP expression
    Depositor
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pZA16

    Plasmid
    #158494
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D481V, no STOP codon
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pZA7

    Plasmid
    #158485
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pZA15

    Plasmid
    #158493
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, no STOP codon
    Promoter
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pDUAL_2_tacI

    Plasmid
    #155174
    Purpose
    see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pDUAL_lacZ

    Plasmid
    #155169
    Purpose
    BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression
    Depositor
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pBICI_2_medium

    Plasmid
    #155167
    Purpose
    see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. pBICI_strong

    Plasmid
    #155165
    Purpose
    BaSP and CuCBP co-expression: strong RBS controls BaSP expression
    Depositor
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pBICI_medium

    Plasmid
    #155164
    Purpose
    BaSP and CuCBP co-expression: medium RBS controls BaSP expression
    Depositor
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  17. pBICI_weak

    Plasmid
    #155163
    Purpose
    BaSP and CuCBP co-expression: weak RBS controls BaSP expression
    Depositor
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. pZA6

    Plasmid
    #158484
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, STOP codon
    Promoter
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. pZA14

    Plasmid
    #158492
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon
    Depositor
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    D481V, no STOP codon
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. pZA28

    Plasmid
    #158506
    Purpose
    In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.
    Depositor
    Insert
    ZAR1-4xMyc (ZAR1 Mustard Weed)
    Use
    Tags
    4xMyc
    Expression
    Plant
    Mutation
    L17E, D489V
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 11351 - 11400 of 23588 results