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myc-Raptor

Plasmid

#1859

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Raptor (RPTOR Human)

Tags

Myc

Expression

Mammalian

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Martine Roussel, Peter ten Dijke

Publication

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Expression

Mammalian

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pCMV(MinDis).iGluSnFR

Plasmid

#41732

Purpose

Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Insert

GltI based Glutamate Biosensor

Use

Glutamate biosensor

Tags

Myc, PDGFR transmembrane helix (aa 513-561), and …

Expression

Mammalian

Mutation

cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

Promoter

CMV

Available since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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AAV-GFP/Cre

Plasmid

#49056

Purpose

Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

Depositor

Fred Gage

Publication

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, Myc, and NLS

Expression

Mammalian

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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FH-TET1-pEF

Plasmid

#49792

Purpose

This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

Depositor

Anjana Rao

Publication

Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

Insert

Tet methylcytosine dioxygenase 1 (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF-1alpha

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pcDNA3-EGFP-Rac1-T17N

Plasmid

#12982

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 DN (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Publication

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pAAV-hSyn-EGFP

Plasmid

#50465

Purpose

EGFP under the control of human synapsin promoter

Depositor

Bryan Roth

Has service

AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV11, AAV11 trial size, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial size

Insert

EGFP

Use

AAV

Tags

N/A

Promoter

human Synapsin 1

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

222 Articles

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pLM-CMV-R-Cre

Plasmid

#27546

Purpose

Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

Depositor

Michel Sadelain

Publication

Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

Insert

mCherry_P2A_Cre recombinase

Use

Cre/Lox and Lentiviral

Promoter

CMV

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Publication

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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pLenti PGK Hygro DEST (w530-1)

Plasmid

#19066

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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WWP-Luc (p21/WAF1 promoter)

Plasmid

#16451

Depositor

Bert Vogelstein

Publication

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 promoter (CDKN1A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Publication

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Publication

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Tags

GST

Expression

Bacterial

Available since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCIBN(deltaNLS)-pmGFP

Plasmid

#26867

Purpose

Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

Depositor

Chandra Tucker

Publication

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pSLCAR-CD19-28z

Plasmid

#135991

Purpose

Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Publication

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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Cerulean

Plasmid

#15214

Depositor

Dave Piston

Publication

Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

Insert

monomeric Cerulean

Expression

Mammalian

Mutation

A206K

Available since

June 22, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pcDNA3 Connexin43-GFP-APEX2

Plasmid

#49385

Purpose

Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

Depositor

Alice Ting

Publication

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Connexin43 - EGFP - APEX2

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pCI-His-hUbi

Plasmid

#31815

Depositor

Astar Winoto

Publication

Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

Insert

Ubiquitin (UBB Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pcDNA3.1/Puro-CAG-ASAP1

Plasmid

#52519

Purpose

For mammalian expression of the fluorescent voltage sensor ASAP1

Depositor

Michael Lin

Publication

St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

Insert

Accelerated Sensor of Action Potentials 1

Expression

Mammalian

Promoter

CAG

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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