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Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FH-TET1-pEF
Plasmid#49792PurposeThis plasmid expresses the full-length human TET1 gene with a Flag and HA tag.DepositorInsertTet methylcytosine dioxygenase 1 (TET1 Human)
TagsFlag and HAExpressionMammalianPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-EGFP
Plasmid#50465PurposeEGFP under the control of human synapsin promoterDepositorHas serviceAAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV11, AAV11 trial size, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial sizeInsertEGFP
UseAAVTagsN/APromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
myc-Raptor
Plasmid#1859DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRaptor (RPTOR Human)
TagsMycExpressionMammalianAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRX-IP-GFP-LC3-RFP
Plasmid#84573PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-GFP/Cre
Plasmid#49056PurposeAdeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.DepositorInsertCre
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP, Myc, and NLSExpressionMammalianPromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-XZ066-EGFRvIII
Plasmid#20737DepositorPublicationInsertEGFRvIII (EGFR Human)
UseRetroviralExpressionMammalianMutationEGFR with a 267 amino acid deletion in the extrac…Available sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLCAR-CD19-28z
Plasmid#135991PurposeModular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralExpressionMammalianPromoterEF1a-shortAvailable sinceJune 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-mCherry-Nanobody
Plasmid#70696PurposeExpresses GST-tagged anti-mCherry Nanobody in E. coli cells.DepositorInsertmCherry-Nanobody
TagsGSTExpressionBacterialAvailable sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBFP2-Nuc
Plasmid#14893PurposeMammalian expression of EBFP2 blue fluorescent protein with NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpEBFP2-Nuc
TagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…Available sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLM-CMV-R-Cre
Plasmid#27546PurposeLentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.DepositorInsertmCherry_P2A_Cre recombinase
UseCre/Lox and LentiviralPromoterCMVAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRac1 DN (RAC1 Human)
TagsEGFPExpressionMammalianMutationT17N Dominant negativeAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WWP-Luc (p21/WAF1 promoter)
Plasmid#16451DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWAF1 promoter (CDKN1A Human)
UseLuciferaseTagsluciferaseExpressionMammalianAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-His-hUbi
Plasmid#31815DepositorAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti PGK Hygro DEST (w530-1)
Plasmid#19066Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, HygroDepositorPublicationTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIBN(deltaNLS)-pmGFP
Plasmid#26867PurposeExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membraneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
TagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cerulean
Plasmid#15214DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmonomeric Cerulean
ExpressionMammalianMutationA206KAvailable sinceJune 22, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 Connexin43-GFP-APEX2
Plasmid#49385PurposeExpresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertConnexin43 - EGFP - APEX2
ExpressionMammalianMutationK14D, W41F, E112K, A134P on soybean APX; improved…PromoterCMVAvailable sinceNov. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tau/pET29b
Plasmid#16316DepositorInserttau (MAPT Human)
ExpressionBacterialAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by