We narrowed to 63,044 results for: cloning

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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GCEPIA1-SNAPER

Plasmid

#172659

Purpose

Detect the real-time ER calcium store and local calcium microdomains in live cells.

Depositor

Pingyong Xu

Publication

Luo et al Biophys Rep (2019) 5, 31–42

Insert

calmodulin

Expression

Mammalian

Mutation

Please see depositor comments

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-BICD2

Plasmid

#164626

Purpose

Expresses BICD2 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

BICD2 (BICD2 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

Plasmid

#162386

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-3-SARS-CoV-2-3CL

Plasmid

#168457

Purpose

Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

Depositor

Alejandro Chavez, David Ho, Sho Iketani

Publication

Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

Insert

SARS-CoV-2 3CL protease (ORF1ab Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

March 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pMOS017: ratiometric pHluorin (cytosolic)

Plasmid

#163052

Purpose

ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TRE:GFP

Plasmid

#163036

Purpose

FLiCRE reporter gene for neuronal expression

Depositor

Alice Ting

Publication

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI647 dCas9-Mxi1 TetR KanMX

Plasmid

#139474

Purpose

Integrates CRISPRi effector and tet-responsive regulator

Depositor

Nicholas Ingolia

Publication

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Inserts

dCas9-Mxi1
Tet Repressor

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1 and pTEF1

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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lenti-CDDR

Plasmid

#149355

Purpose

CRISPR/Cas9-based dual-fluorescent DSB repair reporter

Depositor

Tarek Abbas

Publication

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

CDDR Reporter
Blasticidin resistance

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a and hPGK

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP3

Plasmid

#158354

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP3 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAtetO_6xHis-Klentaq

Plasmid

#157862

Purpose

Plasmid for the aTc-inducible expression of the Klentaq

Depositor

Andrew Ellington

Publication

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

Klentaq

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-MBP-SCov2-nsp12

Plasmid

#154759

Purpose

Expression of SARS-CoV-2 nsp12

Depositor

Patrick Cramer

Publication

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Insect

Promoter

Polyhedron

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-GGamma1-GFP2

Plasmid

#140989

Purpose

Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma1-GFP2 (GNGT1 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR207 SARS-CoV-2 NSP3_nostop

Plasmid

#149306

Purpose

Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pmCherry-C1ab-Prkd1

Plasmid

#139314

Purpose

Diacylglycerol biosensor

Depositor

Gerry Hammond

Publication

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)

Expression

Mammalian

Mutation

T208A, N313D

Available since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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S1m tagged DANCR

Plasmid

#138585

Purpose

For in vivo tagging

Depositor

Yin-Yuan Mo

Publication

Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

Insert

DANCR (DANCR Human)

Use

Lentiviral

Tags

S1m tag

Promoter

MSCV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRCH

Plasmid

#136920

Purpose

Expresses BE4max-NRCH in mammalian cells

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-H2B-PAmCherry

Plasmid

#133419

Purpose

Fluorescent reporter for calcium imaging and photoactivation

Depositor

Tim Holy

Publication

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mARG1 cassette 1

Plasmid

#134343

Purpose

Mammalian acoustic reporter gene 1 cassette 1 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Publication

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 1

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMTL5

Plasmid

#127967

Purpose

DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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