We narrowed to 12,348 results for: SHA;

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qTAG-C-3xHA-Zeo

Plasmid

#207740

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-3xHA

Plasmid

#207707

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pColE1_sgRNA_T2_AmpR

Plasmid

#119159

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T3_AmpR

Plasmid

#119160

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T1_AmpR

Plasmid

#119158

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt10B-V5

Plasmid

#35940

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10B (WNT10B Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAJM.944

Plasmid

#108523

Purpose

PCin-YFP Reporter. OHC14 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCin-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagB shRNA #2

Plasmid

#26628

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
Wnt3A STOP

Plasmid

#35871

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR11 mSTIM1 myc

Plasmid

#17733

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM1 (Stim1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
Gq-sensor[mTq2-mNG]

Plasmid

#98884

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mTq2-mNG

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.713

Plasmid

#108514

Purpose

PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PLuxB-YFP

Expression

Bacterial

Available Since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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VZV ORF47

Plasmid

#26698

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

ORF47 (ORF47 Varicella Zoster Virus (HHV-3))

Tags

HA

Expression

Mammalian

Mutation

A 3B3 epitope tag (DQRQYGDVFKGD) from the VZV gB …

Available Since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only
pSY14-ptb

Plasmid

#90490

Purpose

pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

sceC

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2_171

Plasmid

#199103

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pWKS1 ORI plus NGS barcode 17, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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psAAVS1-T2

Plasmid

#60543

Purpose

GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

Depositor

William Simonds

Article

Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

Insert

ProtoSpacer AAVS1-T2 (AAVS1 Human)

Use

CRISPR; Kan

Expression

Mammalian

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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BLING 1

Plasmid

#171647

Purpose

Expresses bioluminescent glutamate indicator in mammalian cells

Depositor

Assaf Gilad

Article

Petersen et al ACS Synth Biol. 2023 Jul 14. doi: 10.102

Insert

BLING 1

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLN377 (FuGW-S(SP1)p-mKate2 )

Plasmid

#105176

Purpose

synthetic promoter SP1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(SP1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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PP255

Plasmid

#78934

Purpose

Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP NFAT5 DBD DM

Plasmid

#14113

Depositor

Anjana Rao

Article

Jauliac et al Nat Cell Biol. 2002 Jul . 4(7):540-4.

Insert

NFAT5 (NFAT5 Human)

Tags

EGFP

Expression

Mammalian

Mutation

DNA binding domain aa 175 to 471, inactive allele

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only