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We narrowed to 14,003 results for: flag

Showing: 11851 - 11900 of 14003 results

  2. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid
    #210710
    Purpose
    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
    Use
    AAV and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/N580A
    Promoter
    M11
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210710-plasmid-map-sequence-id-416264

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  3. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222502
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222502-plasmid-map-sequence-id-441221

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  10. FCM101

    Plasmid
    #194298
    Purpose
    E. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6
    Depositor
    Shohei Koide
    Article
    Albert et al Nat Commun. 2022 Oct 11;13(1):5998. doi:
    Insert
    FCM101
    Use
    Tags
    His tag, FLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    194298-plasmid-map-sequence-id-381074

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  11. ZMYND8-dTAG

    Plasmid
    #190617
    Purpose
    Lentiviral expression plasmid of human ZMYND8 fused with FKBP12G36V for dTAG-mediated degradation
    Depositor
    Junwei Shi
    Article
    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1
    Insert
    ZMYND8 (ZMYND8 Human)
    Use
    Lentiviral
    Tags
    FKBP12_G36V and Flag, 2xHA
    Expression
    Mammalian
    Mutation
    synonymous mutation for sgRNA resistance at aa214…
    Promoter
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    190617-plasmid-map-sequence-id-373000

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  12. pLenti-nSpCas9-TadA8e

    Plasmid
    #209044
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8e-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209044-plasmid-map-sequence-id-413636

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  13. LV-AR-C619Y

    Plasmid
    #171222
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-C619Y (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-C619Y
    Promoter
    EF-1a
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171222-plasmid-map-sequence-id-336120

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  16. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

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  17. pMX-53BP1(1-1710) 3LA

    Plasmid
    #185698
    Purpose
    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    L175A, L517A, and L693A
    Promoter
    SV40
    Available since
    July 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185698-plasmid-map-sequence-id-365946

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  18. pMX-53BP1(1-1710) 3LA7A

    Plasmid
    #185707
    Purpose
    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    T302A, S452A, S523A, S543A, S625A, S784A, and S89…
    Promoter
    SV40
    Available since
    July 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185707-plasmid-map-sequence-id-365947

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  21. pTUU

    Plasmid
    #159749
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159749-plasmid-map-sequence-id-313818

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  22. Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

    Plasmid
    #208039
    Purpose
    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    PML (isoform IVa; 3MAS) (PML Human)
    Use
    Lentiviral
    Tags
    FLAG, N-terminal Split-TurboID
    Expression
    Mutation
    3MAS mutant: K65R, K160R and K490R; mutated SIM
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208039-plasmid-map-sequence-id-410207

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  31. pCB268

    Plasmid
    #53363
    Purpose
    pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.
    Depositor
    Alexander Varshavsky
    Article
    Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:
    Inserts
    DHFR (Dhfr Mouse)
    Ub
    Aβ42 (APP Human)
    Use
    Tags
    13xMyc, Flag, and HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. cDNA5-FRT/TO-APT2-LAMA-F98

    Plasmid
    #234427
    Purpose
    Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98
    Depositor
    William Fuller
    Article
    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:
    Insert
    APT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position F98 (LYPLA2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234427-plasmid-map-sequence-id-470107

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  50. pcDNA5-FRT/TO-APT2-LAMA-G97

    Plasmid
    #234426
    Purpose
    Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97
    Depositor
    William Fuller
    Article
    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:
    Insert
    APT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97 (LYPLA2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234426-plasmid-map-sequence-id-470093

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Showing: 11851 - 11900 of 14003 results