We narrowed to 10,029 results for: CAG

Showing: 4741 - 4760 of 10029 results

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P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Publication

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMU-TLEr

Plasmid

#61187

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgBLM10

Plasmid

#127643

Purpose

Knock-out of human BLM

Depositor

Nicolas Manel

Publication

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 sgRNA (IRF3 Human)

Use

Lentiviral

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Plasmid

#222994

Purpose

Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Publication

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRb1/Cre

Plasmid

#89647

Purpose

Expresses an Rb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Rb1 (Rb1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Publication

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Promoter

U6

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXPR003-sgTP53-2

Plasmid

#118020

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-2 (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

Plasmid

#84249

Purpose

Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgSV40

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE Doc2A-GFP KI

Plasmid

#131478

Purpose

Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Doc2a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDD428

Plasmid

#202081

Purpose

Cas9/sgRNA plasmid targeting the C-terminus of Myh9

Depositor

Daniel Dickinson

Publication

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))

Use

CRISPR

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA3.8

Plasmid

#121958

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, short version

Expression

Mammalian

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3050(gRNA XII-5)

Plasmid

#73292

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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shCDK4/6(1)

Plasmid

#73554

Purpose

vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shRNA against CDK4 + shRNA against CDK6

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

Plasmid

#115518

Purpose

Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.

Depositor

Catherine Verfaillie

Publication

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

eGFP

Use

AAV; Donor plasmid for recombinase-mediated casse…

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-Nono-sh1

Plasmid

#127650

Purpose

Knock-down of human NONO

Depositor

Nicolas Manel

Publication

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO shRNA (NONO Human)

Use

Lentiviral

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217342

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAID5.3-C

Plasmid

#145813

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PX458_CREB1_2

Plasmid

#64940

Purpose

Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPB_2

Plasmid

#64047

Purpose

Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Promoter

U6

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pL1M-pMtRC-MtLAP1-Adh-R1

Plasmid

#170790

Purpose

A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies

Depositor

Jeremy Murray

Publication

Ruan et al Plants (Basel). 2021 Mar 23;10(3). pii:

Insert

MtLAP1 (LOC11412013 Medicago truncatula)

Expression

Bacterial and Plant

Promoter

Medtr4g059670(MtRC)

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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