We narrowed to 59,446 results for: cloning

1. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

   Plasmid

   #111818

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   Mef2c (Mef2c Mouse)
   Tdtomato
   Gata4 (Gata4 Mouse)
   Tbx5
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVITRO1-102.1F10-IgM/λ

   Plasmid

   #61880

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Expression

   Mammalian

   Promoter

   mEF1 Prom and rEF1 Prom

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-dV-IgG1/λ

   Plasmid

   #52214

   Purpose

   Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin gamma 1 constant region
   Human immunoglobulin lambda constant region
   

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-102.1F10-IgA1/λ

   Plasmid

   #50370

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Expression

   Mammalian

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. peSCBE3-NG-Hypa

   Plasmid

   #218165

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVITRO1-102.1F10-IgG1/λ

   Plasmid

   #50366

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Expression

   Mammalian

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. peSCBE3-NG

   Plasmid

   #218163

   Purpose

   This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

   Plasmid

   #154196

   Purpose

   Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sgRNA-BC1-A, sgRNA-BC1-C
   

   Use

   Lentiviral; Catch barcode activation

   Expression

   Mammalian

   Promoter

   hU6 and mU6

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVITRO1-102.1F10-IgG4/λ

   Plasmid

   #50369

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Expression

   Mammalian

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid

    #154197

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC2-A, sgRNA-BC2-C
    

    Use

    Lentiviral; Catch barcode activation

    Expression

    Mammalian

    Promoter

    hU6 and mU6

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVITRO1-102.1F10-IgA2/λ

    Plasmid

    #50371

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVITRO1-102.1F10-IgG2/λ

    Plasmid

    #50367

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-102.1F10-IgG3/λ

    Plasmid

    #50368

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2R3a-VenusYFP-3AT

    Plasmid

    #71269

    Purpose

    Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    VenusYFP
    

    Use

    Gateway

    Tags

    4xGly linker and T3A pea Pisum sativum ribulose-1…

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR8/GW/TOPO-ERF

    Plasmid

    #98626

    Purpose

    Gateway entry TOPO TA cloning vector ERF

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ERF (Erf Mouse)
    

    Use

    Topo ta cloning vector

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid

    #204215

    Purpose

    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    Cas13bt3 and U6.SapI sgRNA cloning site
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimisation by GenScript tool

    Promoter

    CMV/U6

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2R3a-3xVenusYFP-OcsT

    Plasmid

    #71271

    Purpose

    Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    3 times VenusYFP
    

    Use

    Gateway

    Tags

    octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR8/GW/TOPO-ETV1

    Plasmid

    #98623

    Purpose

    Gateway entry TOPO TA cloning vector for ETV1

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ETV1 (Etv1 Mouse)
    

    Use

    Topo ta cloning vector

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. peSCBE3-NG-HF1

    Plasmid

    #218164

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR8/GW/TOPO-ETV6

    Plasmid

    #98625

    Purpose

    Gateway entry TOPO TA cloning vector for ETV6

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ETV6 (Etv6 Mouse)
    

    Use

    Topo ta cloning vector

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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