We narrowed to 16,081 results for: nes

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SpCas9

Plasmid

#188489

Purpose

Expresses FLAG tagged SpCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC-ARS-stacked-NeoR-mNeonGreen

Plasmid

#101249

Purpose

Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMD1_sgRNA_1

Plasmid

#74180

Purpose

lentiviral vector expressing sgRNA targeting PSMD1

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMD1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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FXYD2-luc

Plasmid

#31056

Depositor

Gerhart Ryffel

Article

Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

Insert

FXYD2 (FXYD2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

FXYD2 promoter from -2382 to -1

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

Plasmid

#26577

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pRU293

Plasmid

#167688

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU54

Plasmid

#167680

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-NRASG12D-BFP

Plasmid

#236202

Purpose

Expresses NRASG12D in mammalian cells

Depositor

Michael Heuser

Article

Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12D

Promoter

MSCV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TEK-V5/HIS

Plasmid

#205611

Purpose

Expression of human TEK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TEK receptor tyrosine kinase (TEK Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-tTRE-scFv-SunTag

Plasmid

#193138

Purpose

Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

Depositor

Brad Rosenberg

Article

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

scFv-SunTag

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-msfGFP

Plasmid

#180328

Purpose

mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEMPTY::sgRNA2

Plasmid

#165459

Purpose

Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

Depositor

Inigo Lasa

Article

Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

Insert

sgRNA2

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

SP01

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF1 shRNA

Plasmid

#118345

Purpose

This plasmid expresses shRNA against hHSF1.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 shRNA (HSF1 Human)

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1deltaTMD

Plasmid

#108132

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-521)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf166

Plasmid

#12854

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf166

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pBBR1.cas9-NT

Plasmid

#197871

Purpose

Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

Depositor

Baojun Wang

Article

Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

Inserts

trancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Myh6-Cas9

Plasmid

#109037

Purpose

Cardiac-specific overexpression of Cas9

Depositor

Eva van Rooij

Article

Johansen et al Circ Res. 2017 Oct 27;121(10):1168-1181.

Insert

Cas9

Tags

3XFlag

Expression

Mammalian

Promoter

alpha MHC

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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p70rg

Plasmid

#17827

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only