164,728 results

1. pLX304-IRES-Td

   Plasmid

   #176296

   Purpose

   pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-SpCas9-GFP-U6-gRNA

   Plasmid

   #79144

   Purpose

   All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

   Depositor

   Jizhong Zou

   Article

   Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH2971

   Plasmid

   #100955

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLBS-CAG-TOMM-QUE37C

   Plasmid

   #191524

   Purpose

   ATP measurement, mitochondria outer membrane, lentivirus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   QUEEN-37C
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG26

   Plasmid

   #226743

   Purpose

   Expresses a reporter for a mitochondrial intermembrane space protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MICU1 (targeting sequence) (MICU1 Human)
   

   Use

   Lentiviral

   Tags

   GFP1-10

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-MCS-WPRE-UbC-mCherry

   Plasmid

   #211799

   Purpose

   Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Inserts

   multiple cloning site
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

   Plasmid

   #134868

   Purpose

   Expresses EGFP-tagged lysosomal marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PJEBAN3

   Plasmid

   #203682

   Purpose

   Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

   Depositor

   Tine Rask Licht

   Article

   Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

   Insert

   Yellow Fluorescent Protein (YFP)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Myo 1c

   Plasmid

   #134832

   Purpose

   Expression of rat Myo 1c (Myr 2) in mammalian cells. N-terminal GFP-tag.

   Depositor

   Martin Bähler

   Article

   Ruppert et al J Cell Sci. 1995 Dec;108 ( Pt 12):3775-8

   Insert

   Myo 1c (rat) with 3' UTR (Myo1c Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191796

    Purpose

    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13X
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    R84A, H89A, R739A, R740A, H745A, H746A, H747A

    Promoter

    CBh, SV40, hU6

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLYBL-TO-hNIL-BSD-mApple

    Plasmid

    #124230

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Insert

    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-3XMyc-EGFP-OMP25

    Plasmid

    #83355

    Purpose

    For tagging mitochondria with Myc epitopes (Control-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XMyc-EGFP-OMP25
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Use

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-drebrin

    Plasmid

    #40362

    Depositor

    Phillip Gordon-Weeks

    Article

    Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

    Insert

    drebrin E2 (DBN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pET-28a(+)-FGF1-4X

    Plasmid

    #120282

    Purpose

    Produce human recombinant FGF1-4X in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Basic fibroblast growth factor 1 mutant
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0974

    Plasmid

    #141774

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GFI1B (GFI1B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 Flag MKK7B2Jnk3a2

    Plasmid

    #19729

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk3a2 (Map2k7, MAPK10 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pMEXneo-TrkB

    Plasmid

    #190203

    Purpose

    To express the Human TrkB receptor under the Moloney mouse sarcoma virus LTR

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkB (NTRK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney mouse sarcoma virus right LTR

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF2457

    Plasmid

    #143189

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-Coff/Fon-oScarlet (AAV8)

    Viral Prep

    #137138-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-oScarlet (#137138). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-oScarlet plasmid DNA. EF1a-driven, Flp-dependent expression of oScarlet (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    oScarlet (Flp-dependent)

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only