We narrowed to 9,802 results for: otos

1. pGEX6P1-human LIC1 C-terminal half

   Plasmid

   #74599

   Purpose

   used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
   

   Use

   Tags

   N-terminal GST and Strep II

   Expression

   Bacterial

   Mutation

   amino acids 389-523 of LIC1

   Promoter

   T7

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-MCU-tDimer

   Plasmid

   #181865

   Purpose

   Expresses tDimer-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

   Insert

   mitochondrial calcium uniporter (Mcu Mouse)
   

   Use

   AAV and Adenoviral

   Tags

   tDimer

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP Kind2 S159/181/666A

   Plasmid

   #105310

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666A

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. KLC1-Myc

   Plasmid

   #166962

   Purpose

   Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KLC1 (Klc1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-KIF5A

   Plasmid

   #166958

   Purpose

   Expresses HA-tagged KIF5A protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(gKAT7-5)-PGKpuro2ABFP-W

   Plasmid

   #159287

   Purpose

   Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. 5)

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   guide RNA targeting KAT7 (ID. 5) (KAT7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECE-M2-CDC27 S379A

   Plasmid

   #31662

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   cell division cycle 27 homolog S379A (CDC27 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   changed Serine 379 to Alanine

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-mPC1_4077-eGFP-3HA

   Plasmid

   #108390

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mPC1_4119-3HA

   Plasmid

   #108395

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Use

   Tags

   3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-CDC27 S379A

    Plasmid

    #31674

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    cell division cycle 27 homolog S379A (CDC27 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed Serine 379 to Alanine

    Promoter

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

11. sgYAP-R-1

    Plasmid

    #229445

    Purpose

    knockout of YAP1

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgYAP-R-1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV 9P31-hSyn-Venus-P2A-Tau-RING I18R/M72E

    Plasmid

    #233694

    Purpose

    AAV expression of Venus-P2A-Tau-RING-I18R/M72E from hSyn promoter

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Venus-P2A-Tau-RING I18R-M72E
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid

    #229814

    Purpose

    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    loxp-EGFP-stop-lox
    

    Use

    Cre/Lox; Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

    Plasmid

    #229816

    Purpose

    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    loxp-EGFP-stop-lox
    

    Use

    Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    mScarlet-I
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid

    #229841

    Purpose

    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    loxp-H2B-mCerulean-2xStop-loxP
    

    Use

    Cre/Lox; Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-MST1-7A

    Plasmid

    #229418

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MST1-7A (MST1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    MARK2 phosphosites (T175,T273,S320,S327,T340,T387…

    Promoter

    EFS

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgYAP-R-2

    Plasmid

    #229446

    Purpose

    knockout of YAP1

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgYAP-R-2 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgNF2

    Plasmid

    #229433

    Purpose

    knockout of NF2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgNF2 (NF2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgMARK3-R-2

    Plasmid

    #229438

    Purpose

    knockout of MARK3

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMARK3-R-2 (MARK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. sgYAP-R-3

    Plasmid

    #229447

    Purpose

    knockout of YAP1

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgYAP-R-3 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG-MARK2-K82H-CR

    Plasmid

    #229425

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK2-K82H (MARK2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Kinase-dead mutant K82H; silent mutation CRISPR r…

    Promoter

    EFS

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FLAG-MARK3-GK-CR

    Plasmid

    #229424

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-GK-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Gatekeeper mutation M132G; silent mutation CRISPR…

    Promoter

    EFS

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-MARK2-GK-CR

    Plasmid

    #229423

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK2-GK-CR (MARK2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Gatekeeper mutation M129G; silent mutation CRISPR…

    Promoter

    EFS

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. sgTAZ-R-2

    Plasmid

    #229449

    Purpose

    knockout of WWTR1 (TAZ)

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgTAZ-R-2 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. sgTAZ-R-1

    Plasmid

    #229448

    Purpose

    knockout of WWTR1 (TAZ)

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgTAZ-R-1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sgCDK1-ROSA

    Plasmid

    #229443

    Purpose

    knockout of CDK1

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgCDK1-ROSA (CDK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. sgMARK2-3-3

    Plasmid

    #229442

    Purpose

    knockout of MARK2 and MARK3

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMARK2-3-3 (MARK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. sgMARK2-3-2

    Plasmid

    #229441

    Purpose

    knockout of MARK2 and MARK3

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMARK2-3-2 (MARK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. sgMARK2-3-1

    Plasmid

    #229440

    Purpose

    knockout of MARK2 and MARK3

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMARK2-3-1 (MARK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge