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pSUP202pol4

Plasmid

#67184

Purpose

Conjugative cloning vector

Depositor

Hauke Hennecke, Julia Vorholt

Article

Fischer et al EMBO J. 1993 Jul;12(7):2901-12.

Type

Empty backbone

Use

Conjugative bacterial cloning vector

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-L-mphR

Plasmid

#62560

Purpose

Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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A1ACR1_EYFP_pcDNA3.1

Plasmid

#161025

Purpose

Expresses strongly red-shifted A1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

A1ACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pESC-ARG (V5 and HA)

Plasmid

#222493

Purpose

Co-expression of two genes in yeast with V5 and HA tags and ARG1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-MET (V5 and HA)

Plasmid

#222490

Purpose

Co-expression of two genes in yeast with V5 and HA tags and MET15 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-LYS (V5 and HA)

Plasmid

#222491

Purpose

Co-expression of two genes in yeast with V5 and HA tags and LYS2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-TYR (V5 and HA)

Plasmid

#222492

Purpose

Co-expression of two genes in yeast with V5 and HA tags and TYR1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-THR (V5 and HA)

Plasmid

#222494

Purpose

Co-expression of two genes in yeast with V5 and HA tags and THR1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TC872

Plasmid

#164895

Purpose

TRE::nCre, inducible nCre expression when co-express with rtTA

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

nCre

Expression

Worm

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Zf Slit1a

Plasmid

#61279

Purpose

contains zebrafish Slit1a cDNA

Depositor

Chi-Bin Chien, Lara Hutson

Article

Hutson et al Dev Dyn. 2003 Nov;228(3):358-69.

Insert

Slit1a (slit1a Zebrafish)

Use

In vitro transcription

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pALPS rev

Plasmid

#101327

Purpose

Encodes codon optimized NL4-3 rev

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 rev

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-LEXY (pDN157)

Plasmid

#72658

Purpose

Constitutively nuclear LEXY variant for blocking endogenous, CRM-1-dependent nuclear export with blue light

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-LEXY

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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RAD Module G8-C1

Plasmid

#38208

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

SET Integrase
Bxb1 Excisonase
RESET Integrase

Use

Synthetic Biology; Biobrick cloning vector

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pGMCKq1-10M1-sspBmyc

Plasmid

#27069

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

tetR-PmyctetO-sspB

Expression

Bacterial

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pDUET-ctCPR-A17AMO

Plasmid

#20115

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pILxR5

Plasmid

#149497

Purpose

Broad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-aDTX-lumitoxin

Plasmid

#51686

Purpose

Expression vector for aDTX-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

aDTX-lumitoxin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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PKC zeta dNPS

Plasmid

#21250

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC zeta (Prkcz Rat)

Tags

HA

Expression

Mammalian

Mutation

delta 1-203

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pXWP11-gfp2

Plasmid

#123152

Purpose

pSB1K3 carrying Pecf11-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

Pecf11-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf11

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:lyn-tagRFPt

Plasmid

#192354

Purpose

lyn-tagRFPt expression under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

lyn-tagRFPT

Tags

tagRFPT

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pICH44022

Plasmid

#50330

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, P19 suppressor of gene silencing (Tomato Bushy Stunt Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAB164

Plasmid

#101667

Purpose

Opto-T7RNAP*(563), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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OA-961B

Plasmid

#104967

Purpose

D. suzukii Medea Construct

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Inserts

GFP
dsRed
HR5Ie1

Expression

Insect

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD/CURT_fluoA

Plasmid

#117998

Purpose

Expression of CURT_fluoA controlled by the ARA promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_fluoA

Expression

Bacterial

Promoter

ARA

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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CBLB

Plasmid

#39056

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CBLB (CBLB Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+mTurquoise2-Golgi

Plasmid

#128425

Purpose

Contains insert allowing in-vivo visualization of Golgi apparatus

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

mTurquoise2-Golgi

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJT11F

Plasmid

#165062

Purpose

Introducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

T-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B

Expression

Yeast

Mutation

WT

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CGG-R12-KIRV

Plasmid

#63668

Purpose

CGG-R12-KIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CGG-R12-KIRV RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-CK(0.4)GW

Plasmid

#27226

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

AAV

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDWJ27

Plasmid

#207604

Purpose

GFP-BIB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BIB

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GST-Laforin

Plasmid

#84673

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DTXK-lumitoxin

Plasmid

#51691

Purpose

Expression vector for DTXK-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

DTXK-lumitoxin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-mRFP

Plasmid

#27058

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-mcherry rfp

Expression

Bacterial

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pALPS tat

Plasmid

#101326

Purpose

Encodes codon optimized NL4-3 tat

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 tat

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1(-) TRIM30A

Plasmid

#87081

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30A (Trim30a Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12B

Plasmid

#87079

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12B (Trim5 Mouse)

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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ClhN-4V5-APEX2-URA

Plasmid

#207056

Purpose

For ORF tagging with 4V5-APEX2

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

4xV5-APEX2

Tags

4xV5-APEX2

Expression

Yeast

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJKR-L-ttgR

Plasmid

#62566

Purpose

Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS6-mPSD95a-TS2-HA

Plasmid

#42240

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS2-HA

Expression

Mammalian

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSB4A5 JQ929581

Plasmid

#38168

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 9641 - 9680 of 44063 results

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