We narrowed to 20,258 results for: INO

Showing: 4821 - 4840 of 20258 results

Results per page:

Sort results by:

pXR002: EF1a-dCasRx-2A-EGFP

Plasmid

#109050

Purpose

Catalytically inactive dCasRx with 2A-EGFP

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

dCasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCph8

Plasmid

#22869

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

Insert

pCph8

Use

Synthetic Biology

Expression

Bacterial

Mutation

First 517 amino acids of Cph1 and the last 229 am…

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLR16-Phes*

Plasmid

#98783

Purpose

Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

Depositor

Anat Herskovits

Article

Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

Insert

pheS

Expression

Bacterial

Mutation

A309G

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDule-tfmF A65V S158A

Plasmid

#85484

Purpose

Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A

Tags

None

Expression

Bacterial

Mutation

Y32V F108W Q109M I159M with 65Val and 158Ala

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pY095

Plasmid

#84744

Purpose

Expresses huLbCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
GFP

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-cGAS-HA

Plasmid

#130910

Purpose

Expresses human cGAS-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_816

Plasmid

#201157

Purpose

EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

5x-ALFA tag-dEnAsCas12a-VP64

Use

CRISPR and Lentiviral

Mutation

D908A

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b Adrm1

Plasmid

#19423

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

His

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pMRS-XylS-dCas9

Plasmid

#220196

Purpose

mini-Tn7 dCas9 expression vector - XylS/pM promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

XylS/pM

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b_PstS_1

Plasmid

#78198

Purpose

Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A197C)

Expression

Bacterial

Mutation

A197C (please see depositors comment below)

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Neo-Bam p53 wt

Plasmid

#16434

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pLR5-CAG-hMCP-mScarlet-I-NLS

Plasmid

#174878

Purpose

PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

MCP-mScarlet-I-NLS

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Myc-14-3-3ζ

Plasmid

#48798

Purpose

expresses human myc-14-3-3ζ in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Insert

YWHAZ (YWHAZ Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX_TRE3G_DD::mCherry

Plasmid

#108679

Purpose

Tunable system for controlled gene expression and protein stability in mammalian cells

Depositor

Lucia Marucci

Article

Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

Insert

Destabilizing Domain (DD) mCherry fusion protein

Use

Lentiviral

Tags

DD::mCherry fusion protein

Expression

Mammalian

Promoter

Doxycycline inducible

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGN-HCF-1-N1011

Plasmid

#92320

Purpose

N-term 1-1011 of HCF-1 protein

Depositor

Winship Herr

Article

Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

Insert

HCF-1 (HCFC1 Human)

Tags

HA

Expression

Mammalian

Mutation

AA 2-1011

Promoter

CMV

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
C3 luc (T1 del)

Plasmid

#11358

Depositor

Donald McDonnell

Article

Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

Insert

C3 promoter (C3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.Halo-LDLR

Plasmid

#162717

Purpose

Mammalian expression of human LDLR

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

LDLR (LDLR Human)

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Mutation

*

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge