We narrowed to 15,673 results for: NOL

1. pAGT10446

   Plasmid

   #192353

   Purpose

   L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   TTCG_Stop-t35S_CGCT
   

   Expression

   Plant

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Marathon-RT for Split-PE (pEH59)

   Plasmid

   #190108

   Purpose

   Expresses Marathon-RT (human codon optimization)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL3.7 Scr shRNA

   Plasmid

   #59299

   Purpose

   non-targeting shRNA

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

   Insert

   Scr shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pRC319

   Plasmid

   #61272

   Purpose

   Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJBL701

   Plasmid

   #140371

   Purpose

   Expresses TetR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-TetR-T
   

   Tags

   6XHis

   Expression

   Bacterial

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2191

   Plasmid

   #67538

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSET-dCas9-actII-4-NT-S1

   Plasmid

   #110185

   Purpose

   Repression of gene expression in Streptomyces by CRISPRi

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Inserts

   dCas9
   sgRNA
   

   Use

   CRISPR; I

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   J23119 and ermE*p

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-NHERF1

   Plasmid

   #31650

   Depositor

   Edward Weinman

   Article

   Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

   Insert

   solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
   

   Tags

   His & S tag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETNb-nALFA

   Plasmid

   #159988

   Purpose

   For the protein expression with ALFA tag at N-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

   Expression

   Bacterial

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTR47m4

    Plasmid

    #104068

    Purpose

    Luciferase Reporter for Formaldehyde Sensing

    Depositor

    David Liu

    Article

    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

    Inserts

    xluxAB
    frmR
    frm Promoter m4
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Mutation

    23r and 25th nucleotides in sequence mutated from…

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

    Plasmid

    #48297

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    StrepII and msfGFP

    Expression

    Insect

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB2189

    Plasmid

    #67532

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSIIbsr-PKG-hNo.7

    Plasmid

    #175812

    Purpose

    The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

    Depositor

    Michiyuki Matsuda

    Article

    Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

    Insert

    PKG #7
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTK095

    Plasmid

    #59560

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
    

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTW170b2

    Plasmid

    #136929

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gVI
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET51b(+)_MBP-nLucCP-67/68

    Plasmid

    #113922

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-67/68
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cdk9-Halo donor

    Plasmid

    #113113

    Purpose

    Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Cdk9 donor region including homology arms and HaloTag
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PE-Cas9

    Plasmid

    #174851

    Purpose

    Express PE-Cas9 in mammalian cells

    Depositor

    Wen Xue

    Article

    Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    PE-Cas9
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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