We narrowed to 19,001 results for: Tec

1. pENTR-L3-SpCas9-HF1-2A-EGFP-L2

   Plasmid

   #141048

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   SpCas9-HF1-2A-EGFP
   

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYC3.60-C1

   Plasmid

   #67899

   Purpose

   Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

   Insert

   YC3.60
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-Flp-DOG

   Plasmid

   #80084

   Purpose

   Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   Flp-DOG
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFYF1328 EGFP Site#3

   Plasmid

   #47513

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 3
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH-dCas9-PBE

   Plasmid

   #98162

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-dCas9-UGI-NLS
   

   Use

   Rice

   Expression

   Plant

   Promoter

   Ubi

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. CP1028-CBEmax

   Plasmid

   #119802

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   rApobec1-nSpCas9 (CP1028) -UGI-UGI
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPANI2A2E2-Crimson

   Plasmid

   #193758

   Purpose

   Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

   Insert

   E2-Crimson
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PANI2 synthetic promoter carrying binding sites f…

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-5xUASG-iRFP-B3-timestamp-A

   Plasmid

   #159925

   Purpose

   timestamp Template A inserted into the 3’ UTR of iRFP, in a second generation lentiviral backbone with the Vivid promoter, with RNA barcode CTG. AKA 149B3.

   Depositor

   Fei Chen

   Article

   Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

   Insert

   iRFP-Timestamp A
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT371

   Plasmid

   #125612

   Purpose

   Expresses CDA1-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   CDA1-BE4max-NG
   

   Expression

   Mammalian

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ210

    Plasmid

    #109329

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Expression

    Plant

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGY53

    Plasmid

    #221709

    Purpose

    Empty Cas12a vector for multiplex gene editing in fungi

    Depositor

    Kyle Pomraning

    Article

    Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

    Type

    Empty backbone

    Use

    CRISPR; Aspergillus

    Expression

    Bacterial

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

    Plasmid

    #169586

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gata3-T2A-H2B-eGFP donor

    Plasmid

    #113119

    Purpose

    Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Gata3 donor region including homology arms and T2A-H2B-eGFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Fgfr2-T2A-H2B-Halo donor

    Plasmid

    #113117

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Fgfr2 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pISFba1

    Plasmid

    #226820

    Purpose

    Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1
    

    Expression

    Bacterial

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pISFba1reRNA

    Plasmid

    #226822

    Purpose

    Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1 none target guide RNA
    

    Expression

    Bacterial

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKB817 (pBra-Hfq)

    Plasmid

    #174661

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

    Depositor

    Ann Hochschild, Katherine Berry

    Article

    Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

    Insert

    Hfq
    

    Tags

    alpha NTD

    Expression

    Bacterial

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PclpB riboswitch GFP pZa

    Plasmid

    #170078

    Purpose

    GFP expression under the control of E. coli clpB promoter engineered with riboregulators

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PclpB

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Cas9-NRRH

    Plasmid

    #136924

    Purpose

    Expresses Cas9-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRRH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-iCre

    Plasmid

    #125577

    Purpose

    Expresses iCre under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    iCre
    

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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