We narrowed to 8,370 results for: construct

1. pFB-GluCl_cryst

   Plasmid

   #31488

   Depositor

   Eric Gouaux

   Article

   Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

   Insert

   C. elegans glutamate-gated chloride channel alpha, crystallized construct
   

   Use

   Tags

   8x-His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pRET.IS.IRES-EGFP N2

   Plasmid

   #1834

   Depositor

   Philip Leder

   Article

   Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

   Type

   Empty backbone

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pexA2_2hb_ruleB

   Plasmid

   #89670

   Purpose

   Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the two-helix-bundle shown in figure 2E,F,G of associated article.

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   2hb_ruleB
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRET.IIS.IRES-EGFP

   Plasmid

   #1838

   Depositor

   Philip Leder

   Article

   Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

   Type

   Empty backbone

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pRW398-URA3-BXB1-LP

   Plasmid

   #235977

   Purpose

   Plasmids for generation of donor DNA for construction of a BXB1-compatible landing pad p1 with URA3 selection marker.

   Depositor

   Chang Liu

   Article

   Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

   Insert

   URA3
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRW402-P1int-BXB1

   Plasmid

   #235979

   Purpose

   Plasmid used for constructing donor DNA for BXB1-compatible p1 integration. You can insert your GOIs into this plasmid by using Golden Gate Assembly.

   Depositor

   Chang Liu

   Article

   Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. psLIB TRE mTFP1-actin

   Plasmid

   #229970

   Purpose

   encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   mTFP1-actin
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLAP

   Plasmid

   #200786

   Purpose

   Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Type

   Empty backbone

   Use

   Tags

   CLIPf and SNAPf-6xHis-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rab11A[8-175]-His-C Q70L

   Plasmid

   #228042

   Purpose

   Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).

   Depositor

   Wade Harper

   Insert

   Rab11A[8-175]-His-C Q70L (RAB11A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q70L

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. L0-T3 mTurq-tUBQ10

    Plasmid

    #195886

    Purpose

    Level 0 mTurq-tUBQ10 for integrase target construction

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    mTurq-UBQ10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYTK001_E2Aq(2nd)

    Plasmid

    #190911

    Purpose

    2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    E2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYTK001_O2Aq(2nd)

    Plasmid

    #190918

    Purpose

    2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    O2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYTK001_P2Aq(1st)

    Plasmid

    #190912

    Purpose

    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    P2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYTK001_O2Aq(1st)

    Plasmid

    #190917

    Purpose

    2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    O2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYTK001_P2Ab

    Plasmid

    #183084

    Purpose

    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    P2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYTK001_P2Aq(2nd)

    Plasmid

    #183089

    Purpose

    2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    P2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMbd3EosHaloTAF_Hyg

    Plasmid

    #140958

    Purpose

    Targeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    methyl-CpG binding protein 3 (Mbd3 Synthetic, Mouse)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pChd4EosHaloTAF_Puro

    Plasmid

    #172099

    Purpose

    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    chromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-EnRRbpj

    Plasmid

    #160925

    Purpose

    dominant-negative construct to inhibit Notch signaling

    Depositor

    Mark Emerson

    Article

    Chen et al Sci Rep. 2021 Jul 15;11(1):14525. doi: 1

    Insert

    EnRRbpj
    

    Use

    Tags

    Expression

    Mutation

    fused with an engrailed repressor

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPEE36

    Plasmid

    #128376

    Purpose

    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the NEO resistance marker

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    ACT1

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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