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CMV-mito-R-GECO1

Plasmid

#46021

Purpose

Red intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

R-GECO1

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to the mApple-derived anal…

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFPZNF598-9P-9A

Plasmid

#141192

Purpose

Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Tags

GFP

Expression

Mammalian

Mutation

all 3 proline repeats are mutated to Alanine (9xP…

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-full length LIC1

Plasmid

#74601

Purpose

expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 (DYNC1LI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LAMP1-RpHLuorin2

Plasmid

#171720

Purpose

Lysosomal expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLIX405-KANK1

Plasmid

#121984

Purpose

tetracycline-inducible expression of C-terminal GFP tagged KANK1

Depositor

GuangJun Zhang

Article

Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

Insert

KANK1 (KANK1 Human)

Use

Lentiviral

Tags

Emerald GFP

Promoter

Tight TRE promoter

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Stx5L-mCherry

Plasmid

#209844

Purpose

Expresses Stx5L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Wildtype Stx5 (with 55M)

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-REX-GECO1

Plasmid

#61248

Purpose

Expresses REX-GECO1 in neurons

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO1

Use

AAV

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

hSyn

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-P405A/P531A-pcDNA3

Plasmid

#18956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1 KanR foundation lox casette

Plasmid

#132388

Purpose

Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

G418 resistance

Use

CRISPR and Cre/Lox

Expression

Mammalian

Promoter

promoterless

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-ASAP5-Kv2.1-WPRE

Plasmid

#225707

Purpose

Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5-Kv2.1

Use

AAV

Promoter

human synapsin

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-CRY2-CreN

Plasmid

#26888

Purpose

Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2-CreN (cre, CRY2 Mustard Weed)

Expression

Mammalian

Mutation

aa 19-104 of Cre

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

Plasmid

#229792

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-HA-mScarlet3-tdP2A-Frankenbody-FLAG-mEGFP-IRESv4-HaloTag-tdMCP

Plasmid

#241140

Purpose

Expresses Anti-HA frankenbody-mScarlet3, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent HA and FLAG-tagged proteins and MS2-tagged mRNAand MS2-tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-FLAG frankenbody
anti-FLAG frankenbody
tdMCP

Tags

HaloTag, mEGFP, and mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Myc-Idax DBM

Plasmid

#68554

Purpose

This plasmid expresses full length IDAX containing a DNA binding mutation.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

IDAX-DBM (Cxxc4 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

T162A, H164A and Q165A mutations

Promoter

EF1a

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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CMV Flag PAI-RBP

Plasmid

#45452

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP (SERBP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
HA-HIF2alpha-P405A/P531A-pBabe-puro

Plasmid

#19006

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: P405A and P531A

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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