We narrowed to 59,476 results for: cloning

1. pET24c(+)-mtEFTu

   Plasmid

   #30535

   Depositor

   Linda Spremulli

   Article

   Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

   Insert

   Mature bovine mitochondrial elongation factor Tu
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Mature form amino acids 44-452. Mito import seque…

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

2. mTB3-Myc tag

   Plasmid

   #210205

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   Myc-tag
   

   Expression

   Bacterial

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GGE177

   Plasmid

   #120766

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   T1_Xpr2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p667-UBC-AAK1-V5_IDG-K

   Plasmid

   #135257

   Purpose

   Gateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   AAK1-V5 (AAK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-L1-ESYN-R5

   Plasmid

   #32581

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   Enhanced Synapsin Promoter
   

   Use

   Gateway cloning vector

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. MXS_CMV

   Plasmid

   #62417

   Purpose

   MXS_chaining vector with CMV

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   CMV Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MCV

   Plasmid

   #81174

   Purpose

   MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH54

   Plasmid

   #128183

   Purpose

   Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MXS_BiTet

   Plasmid

   #62423

   Purpose

   MXS_chaining vector with BiTet

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   Bidirectional tetracycline inducible promoter
   

   Use

   Synthetic Biology

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. MXS_bGpA

    Plasmid

    #62424

    Purpose

    MXS_chaining vector with bGpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    beta-Globin poly A
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECT3

    Plasmid

    #75450

    Purpose

    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    pdt3
    

    Expression

    Bacterial

    Promoter

    none

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGY001

    Plasmid

    #48866

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRATIO2214

    Plasmid

    #141303

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mCerulean and mScarlet-I

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKER-mKOk

    Plasmid

    #85975

    Purpose

    Expresses mKOk from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mKOk
    

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGGY003

    Plasmid

    #48867

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

    Plasmid

    #164263

    Purpose

    Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Arabinose

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MECP2 - R133C in pENTR

    Plasmid

    #216594

    Purpose

    gateway cloning donor vector containing MECP2 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Mutation

    R133C

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRATIO1267

    Plasmid

    #141301

    Purpose

    Gateway cloning compatible dual bioluminescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    gLUC and redLUC

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. R26P-SA-EGFP-puro

    Plasmid

    #26890

    Depositor

    Rudolf Jaenisch

    Article

    Blelloch et al Proc Natl Acad Sci U S A. 2004 Sep 28. 1

    Insert

    EGFP
    

    Use

    Mouse Targeting

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pOGG216

    Plasmid

    #114002

    Purpose

    Level 2 golden gate cloning vector for medium copy number free-living expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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