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We narrowed to 26,655 results for: Vit;

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Showing: 9921 - 9960 of 26655 results

  7. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid
    #115161
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.
    Inserts
    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    Use
    AAV
    Tags
    hM3Dq-mCherry
    Expression
    Mammalian
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115161-plasmid-map-sequence-id-220939

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  8. HCTR1-DuET

    Plasmid
    #213310
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCTR1 (HCRTR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213310-plasmid-map-sequence-id-430848

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  9. pC1-oROS-HT-CaaX

    Plasmid
    #216417
    Purpose
    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    oROS-HT-CaaX
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216417-plasmid-map-sequence-id-434853

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  15. GIPR-DuET

    Plasmid
    #213252
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GIPR (GIPR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213252-plasmid-map-sequence-id-430802

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  21. CHRM2-DuET

    Plasmid
    #213208
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM2 (CHRM2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213208-plasmid-map-sequence-id-430770

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  22. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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  23. APJ-DuET

    Plasmid
    #213185
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    APJ (APLNR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213185-plasmid-map-sequence-id-430751

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  29. pMIA10.7

    Plasmid
    #214451
    Purpose
    BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.
    Depositor
    Roger Foo
    Article
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Inserts
    Tet-On 3G
    PuroR
    TRE3G CopGFP
    Use
    Bxbi donor
    Tags
    PuroR, T2A, and Tet-On 3G
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214451-plasmid-map-sequence-id-431689

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  33. Talin-CS

    Plasmid
    #83377
    Purpose
    Control FRET probe for measuring tension on Talin molecule
    Depositor
    Martin Schwartz
    Article
    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted at C-terminal o…
    Promoter
    CMV
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    83377-plasmid-map-sequence-id-158762

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  34. ADRB3-DuET

    Plasmid
    #213182
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRB3 (ADRB3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213182-plasmid-map-sequence-id-430749

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  36. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid
    #131312
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    131312-plasmid-map-sequence-id-254654

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  39. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113035-plasmid-map-sequence-id-224728

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Showing: 9921 - 9960 of 26655 results