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pCMV_RTN3E-mScarlet3

Plasmid

#216255

Purpose

mammalian expression vector for RTN3E-mScarlet3

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

reticulon 3 isoform e (RTN3 Human)

Tags

mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Cterminal HA WDR59 pRK5

Plasmid

#46328

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR59 (WDR59 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pEMS2144

Plasmid

#104357

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

Insert

ssAAV-smCBA-EmGFP

Use

AAV

Promoter

smCBA

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pNCST-avGFP

Plasmid

#129508

Purpose

Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

avGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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DYRK1A/pTRE3G-FL-hXIST

Plasmid

#149608

Purpose

Express doxycycline-inducible full length of human XIST cDNA

Depositor

Jeanne Lawrence

Article

Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

Insert

X-inactive specific transcript (XIST Human)

Expression

Mammalian

Promoter

pTRE3G

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBF_HsPepT2

Plasmid

#73944

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT2 (SLC15A2 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pBF_HsPepT1

Plasmid

#73943

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT1 (SLC15A1 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLCKO

Plasmid

#73311

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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p6347 MSCV-CMV-Flag-HA-Brd4-444-722

Plasmid

#31353

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 444-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_nLightG

Plasmid

#187179

Purpose

Expresses green norepinephrine indicator nLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has Service

AAV9

Insert

nLightG

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGAPTrap TALEN 2

Plasmid

#83369

Purpose

Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN2

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGAPTrap-TALEN1

Plasmid

#83368

Purpose

Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN1

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pSIN4-EF2-O2S

Plasmid

#21162

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4, Sox2 (SOX2, POU5F1 Human)

Use

Lentiviral; Reprogramming

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAX198

Plasmid

#173042

Purpose

Custom vector for sgRNA library construction

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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