We narrowed to 41,904 results for: tro

1. pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP

   Plasmid

   #171842

   Purpose

   Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   R108E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mcherry-WIPI2dR125E-cs-TEV-STREP

   Plasmid

   #171843

   Purpose

   Mammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   R125E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-mcherry-WIPI2dK128E-cs-TEV-STREP

   Plasmid

   #171844

   Purpose

   Mammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   K128E

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV.U6gLacZ.EFS-NS.H2B-RFP

   Plasmid

   #170363

   Purpose

   Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS-NS

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. THI ONLY pGAP T-

   Plasmid

   #161503

   Purpose

   Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T-_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2 (non activation control NAC)
   gRNA (Non targeting control NTC)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP, pPOR1, and pTEF2

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

   Plasmid

   #162639

   Purpose

   Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acids K174, R179, and K182 mutated to alani…

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTOPO-HqERV(del5'-LTR)

   Plasmid

   #164089

   Purpose

   transfection of Hq-ERV without 5'-LTR

   Depositor

   Maik Hintze

   Article

   Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

   Insert

   Hq-ERV
   

   Use

   Eukaryotic expression vector

   Tags

   Expression

   Mutation

   deletion of 5'-LTR

   Promoter

   none (5'-LTR deleted)

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTOPO-HqERV(C1-20)

   Plasmid

   #164090

   Purpose

   transfection of Hq-ERV with light-inducible promoter

   Depositor

   Maik Hintze

   Article

   Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

   Insert

   Hq-ERV
   

   Use

   Eukaryotic expression vector

   Tags

   Expression

   Mutation

   5'-LTR replaced with light-regulatable C1-20…

   Promoter

   light-activatable C1-20

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBz172_CsoS2_N1_GFP

   Plasmid

   #140852

   Purpose

   His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeat 1 fused to GFP
   

   Use

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBz175_CsoS2_N4_GFP

    Plasmid

    #140855

    Purpose

    His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 4 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBz173_CsoS2_N2_GFP

    Plasmid

    #140853

    Purpose

    His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 2 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBz174_CsoS2_N3_GFP

    Plasmid

    #140854

    Purpose

    His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 3 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLz26_CsoS2_N1-N2_GFP

    Plasmid

    #140851

    Purpose

    His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeats 1-2 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEBTetBl-CLIP-Centrin3

    Plasmid

    #136855

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEBTetDBl-CLIP-Centrin3

    Plasmid

    #136856

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEBTetDBl-CLIP-FOP

    Plasmid

    #136846

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-FGFR
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEBTetDBl-CLIP-POC5

    Plasmid

    #136836

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-POC5 (POC5 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEBTetBl-CLIP-FOP

    Plasmid

    #136845

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-FGFR
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBTetBl-CLIP-POC5

    Plasmid

    #136835

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-POC5 (POC5 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBTetD-SNAP-POC5

    Plasmid

    #136787

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-POC5 (POC5 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge