We narrowed to 25,322 results for: GRN;

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-CBh-eGFP

Plasmid

#206885

Purpose

cloning backbone for sgRNA using OptScf2

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Type

Empty backbone

Promoter

U6

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro G3BP1-sgRNA

Plasmid

#196197

Purpose

Cas9 + gRNA expression to edit G3BP1 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

G3BP1 (G3BP1 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBx-Spas-sgRNA-Kan

Plasmid

#105233

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCas9/VRQR-sgRNA (BbsI)

Plasmid

#129725

Purpose

Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 FLAG-NGRN-Fc

Plasmid

#115773

Purpose

Expression of the extracellular domain of NGRN with N-terminal FLAG tag and C-terminal hIgG tag.

Depositor

Davide Comoletti

Insert

NGRN (NGRN Human)

Tags

FLAG and hIgG/Fc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro

Plasmid

#167911

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3799-gRNA-Csy4-template

Plasmid

#182827

Purpose

SpCas9 gRNA-Csy4 template

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

SpCas9 gRNA scaffold + Csy4 sequence

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pScalps eSpCas9_Zsgreen gRNA scaffold

Plasmid

#188449

Purpose

Expression of eSpCas9 tagged with Zsgreen

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF1-alpha

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pT4-U6-sgRNA-CMV-EGFP

Plasmid

#239587

Purpose

Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Type

Empty backbone

Use

Sleeping beauty transposone

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ3538 eforRed-targeting sgRNA

Plasmid

#239455

Purpose

guide for eforRed

Depositor

Stanley Qi

Article

Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

Insert

guide targeting eforRed

Use

CRISPR; Cell-free system using bacterial extract

Expression

Bacterial

Promoter

J23119

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pNTI661 pRPR1(TetO)-sgRNA

Plasmid

#139475

Purpose

Vector for tet-regulated sgRNA expression in yeast

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Insert

single guide RNA scaffold

Use

CRISPR

Expression

Yeast

Promoter

pRPR1(TetO)

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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CMVp-dsRed2-Triplex-28-gRNA1-28-pA

Plasmid

#55200

Purpose

Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dsRed2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pUb-Cas9-EGFP_cU6:6-sgRNA

Plasmid

#190597

Purpose

The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

Depositor

Claudia Rueckert

Article

Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

Inserts

Cas9
sgRNA

Use

CRISPR

Tags

EGFP - separated by T2A

Promoter

Aedes aegypti polyubiquitin and Culex quinquefasc…

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-8XPP7

Plasmid

#121940

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-mDNMT1_nick-lenti

Plasmid

#135959

Purpose

S. pyogenes nicking sgRNA for mouse DNMT1

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

mDNMT1_nick

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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