We narrowed to 63,044 results for: cloning
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Plasmid#179543Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertS. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW57-mCherry-2A-BRD4 Iso A
Plasmid#137720PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoformDepositorInsertBRD4 long isoform (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianPromotertight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKRaION2-N1
Plasmid#177802PurposeMammalian expression of green fluorescent potassium indicator KRaION2DepositorInsertKRaION2
ExpressionMammalianPromoterCMVAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-HaloTag9
Plasmid#175543PurposeExpression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cellsDepositorInsertLyn11-HaloTag9
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-BSD
Plasmid#174819PurposeMammalian expression of SpCas9 prime editor 2 with P2A-BSD markerDepositorInsertPE2-P2A-BSD
TagsP2A-BSD and SV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK172-α-syn-TEV-GFP
Plasmid#166671PurposeBacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studiesDepositorInsertmouse synuclein (from V. Lee) (Snca Mouse)
TagsGFPExpressionBacterialMutationadded a TEV cleavage site sequence between synucl…PromoterCMVAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-EB3mCherry_cmv-LifeActGFP
Plasmid#133927PurposeLive dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeletonDepositorInsertEB3-mCherry and Lifeact-GFP (MAPRE3 Human)
ExpressionMammalianAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-RUNX1-P2A-ERG-IMPROVED
Plasmid#154089PurposeImproved version to express RUNX1, P2A, ERG in mammalian cells.DepositorUseLentiviralExpressionMammalianPromoterTet onAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EBFP2(1-10)
Plasmid#165141PurposeExpresses EBFP2(1-10) in mammalian cellsDepositorInsertEBFP2(1-10)
ExpressionMammalianAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTet-O-ATOH1-T2A-PuroR
Plasmid#162342PurposeLentiviral expression of ATOH1 under the control of the TetON promoterDepositorAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNHEJ-recX-sacB
Plasmid#158718PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.DepositorInsertrecX
ExpressionBacterialPromoterPmycAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-PTDSS1-IRES-Puromycin
Plasmid#134667PurposeExpresses EGFP-tagged Mitochondria-associated ER membrane markerDepositorAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mtagBFP2-CaM-uTEVp
Plasmid#163033PurposeFLiCRE protease for neuronal expressionDepositorInsertBFP-CaM-uTEVp
UseAAVExpressionMammalianPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1
Plasmid#158738PurposeMammalian expression of scFLARE1 componentDepositorInsertscFLARE1
UseAAVTagsFlag, V5ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-MCP-huADARE488QT490A
Plasmid#159922PurposeFusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5DepositorAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS004 - pCAGGS-3XFLAG-CTCF-eGFP
Plasmid#156438PurposeFor transient expression of mouse CTCF tagged with eGFPDepositorAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5 CMV 3xFG eIF4G1
Plasmid#158775PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.DepositorInserteIF4G1
Tags3xFlagExpressionMammalianAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0med
Plasmid#153286PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in neuronsDepositorHas serviceAAV2InsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVPromoterhSynAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai3-RLuc8
Plasmid#140975PurposeEncodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai3-RLuc8 (GNAI3 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLAI-Env
Plasmid#133996PurposeEnv gene from HIV-1 LAI in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
ExpressionMammalianPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by