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pEMS2177

Plasmid

#111892

Purpose

High copy number plasmid containing Ple344 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple344 (TUBB3 Human)

Use

Unspecified

Promoter

Ple344 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2175

Plasmid

#111890

Purpose

High copy number plasmid containing Ple342 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

Unspecified

Promoter

Ple342 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2176

Plasmid

#111891

Purpose

High copy number plasmid containing Ple343 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple343 (TUBB3 Human)

Use

Unspecified

Promoter

Ple343 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)mGAT1YFP*

Plasmid

#41665

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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p3E-DysF

Plasmid

#109544

Purpose

Multisite gateway vector for 3' tagging with Dysferlin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dysferlin (dysf Zebrafish)

Use

Zebrafish plasmids

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS1984

Plasmid

#49115

Purpose

AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple253-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

PITX3

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D

Plasmid

#32457

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S129D, S133D, S137D (S3V, G96R, and P108L also pr…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pEnt R3L2 TetO(sh) mCh-Rs1-2

Plasmid

#24417

Purpose

Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1.

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Rs1

Use

Entry vector

Tags

FLAG and mCherry-P2A

Mutation

D100A

Promoter

short TetO, miniCMV

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CaMKKbeta S129A, S133A, S137A

Plasmid

#32454

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

S129A, S133A, S137A

Promoter

CMV

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
J23110-mRFP1-331Bb

Plasmid

#78277

Purpose

mRFP1 behind constitutive promoter J23110 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23110-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2276

Plasmid

#111894

Purpose

High Copy number plasmid containing Ple346 (NEFM MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple346 (NEFM Human)

Use

Unspecified

Promoter

Ple346 (NEFM MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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SIN-CMV-Ca9-WPRE

Plasmid

#87874

Purpose

Transfer plasmid for the production of lentiviral vectors

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

spCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-HA-TSAd-d239-74

Plasmid

#84560

Purpose

mammalian expression of TSAd-d239-74

Depositor

Anne Spurkland

Article

Berge et al PLoS One. 2010 Mar 18;5(3):e9761. doi: 1

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of aa 239-274

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pLCKO_PSMB2_sgRNA_5

Plasmid

#74185

Purpose

lentiviral vector expressing sgRNA targeting PSMB2

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMB2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 Promoter

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-ARF1-DN

Plasmid

#109536

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ARF1 (arf1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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J23112-mRFP1-331Bb

Plasmid

#78278

Purpose

mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23112-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23103-mRFP1-331Bb

Plasmid

#78273

Purpose

mRFP1 behind constitutive promoter J23103 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23103-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23105-mRFP1-331Bb

Plasmid

#78275

Purpose

mRFP1 behind constitutive promoter J23105 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23105-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23101-mRFP1-331Bb

Plasmid

#78272

Purpose

mRFP1 behind constitutive promoter J23101 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23101-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCINeo-hFX(I154F)-HA

Plasmid

#14977

Depositor

Michael Ristow

Article

Schulz et al J Biol Chem. 2006 Jan 13. 281(2):977-81.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Mutation

changed isoleucine 154 to phenylalanine

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only