-
Plasmid#170519PurposePlasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-mCherry
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSGV Mouse Acceptor
Plasmid#128176PurposeA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-MCS-IRES-eGFP-P2A-iCas9
Plasmid#178533PurposeFor genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cellsDepositorInsertiCaspase9 (CASP9 Human)
UseTagsExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lack…PromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
Plasmid#70231PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsG
Plasmid#137924PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP670
Plasmid#79984PurposeMonomeric near-infrared fluorescent protein miRFP670 in bacterial plasmidDepositorInsertmiRFP670
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR26 GFP Dest
Plasmid#74283PurposeGene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF.PGK.GFP
Plasmid#17618DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
SypHer
Plasmid#48250PurposepH sensorDepositorInsertSypHer
UseTagsExpressionMammalianMutationC199SPromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-MPro-eGFP-2
Plasmid#184824PurposeExpresses Mpro-eGFP in mammalian cellsDepositorInsertMpro-eGFP conjugate (M SARS-CoV-2, jellyfish Aequorea Victoria)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alfaAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
-
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorInsertRenilla luciferase
UseTags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Fibrillarin-C1
Plasmid#134539PurposeExpresses Cherry-Fibrillarin fusion proteinDepositorInsertFibrillarin (FBL Human)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)
Plasmid#188380PurposeLentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-off Full length Tau (0N4R, P301L/V337M)
Plasmid#188573PurposeDox-repressible expression of the 0N4R Tau isoformDepositorInsert0N4R Tau (MAPT Human)
UseLentiviralTagsExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptf-Galectin3
Plasmid#64149PurposeExpression in mammalian cellsDepositorInsertGalectin3 (LGALS3 Human)
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-AcrIIA4
Plasmid#113037PurposeExpression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)DepositorInsertSV40 NLS-AcrIIA4
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-pBAD
Plasmid#54800PurposeLocalization: Protein Expression Vector , Excitation: 604, Emission: 659DepositorTypeEmpty backboneUseTags6xHis and mCardinalExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDonor_Tn[himar1C9]
Plasmid#213906PurposeMariner transposon with ATc-inducible himar1C9 transposaseDepositorInsertmariner transposon with cam resistance and ATc-inducible himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(mito-LD)
Plasmid#214421PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-3XHA-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NRAS_p.Q61R
Plasmid#81652PurposeGateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertNRAS (NRAS Human)
UseGateway entry vectorTagsExpressionMutationQ61RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM106B-EGFP
Plasmid#210366PurposeExpresses TMEM106B fused with EGFP in mammalian cellsDepositorInsertTMEM106B (TMEM106B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-S727A-STAT3
Plasmid#71446PurposeRetroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationS727APromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
sAJM.1504
Bacterial Strain#108251PurposeMarionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10BDepositorBacterial ResistanceChloramphenicolAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins
Pooled Library#101929PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV9)
Viral Prep#104491-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-tdMCP-msfGFP(V206K)-GBI
Plasmid#205163Purposeexpress tdMCP-msfGFP(V206K)-GBIDepositorInserttdMCP-msfGFP(V206K)-GBI
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only