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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,812 results for: cloning

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Showing: 5221 - 5240 of 63812 results

  1. dCas9-CBP FL

    Plasmid
    #179550
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179550-plasmid-map-sequence-id-354775

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  2. dCas9-CBP core

    Plasmid
    #179543
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179543-plasmid-map-sequence-id-354769

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  5. pCMV-PE2-P2A-BSD

    Plasmid
    #174819
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-BSD
    Tags
    P2A-BSD and SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174819-plasmid-map-sequence-id-343617

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  7. pCMV-EB3mCherry_cmv-LifeActGFP

    Plasmid
    #133927
    Purpose
    Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton
    Depositor
    Frank Bradke, Sebastián Dupraz
    Publication
    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
    Expression
    Mammalian
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    133927-plasmid-map-sequence-id-263527

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  10. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid
    #154429
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Depositor
    David Liu
    Publication
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154429-plasmid-map-sequence-id-304585

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  20. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid
    #140975
    Purpose
    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphai3-RLuc8 (GNAI3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140975-plasmid-map-sequence-id-300658

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Showing: 5221 - 5240 of 63812 results