We narrowed to 7,746 results for: pac

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pAAV-TREtight-mTagBFP2-B19G

Plasmid

#100799

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)

Use

AAV

Expression

Mammalian

Promoter

TREtight

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-PH-PLCD1

Plasmid

#183675

Purpose

PtdIns(4,5)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLCD1 (PLCD1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 11-140

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1_puro_shNRF2-1

Plasmid

#230086

Purpose

shRNA silencing human NRF2 gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.1-ABE8e

Plasmid

#185668

Purpose

Expresses eNme2-T.1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EFSp-GFP-Empty

Plasmid

#174171

Purpose

Control lentiviral vector expressing GFP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pKL038 Lenti U6 dCas12a gRNA Puro mCherry

Plasmid

#195546

Purpose

dCas12a gRNA expression backbone

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti U6- empty cassette_Direct repeat_puro_mcherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP

Plasmid

#181906

Purpose

Fluorescently tagged dead Cas9

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-hPMLIII

Plasmid

#181903

Purpose

Fluorescently tagged PMLIII

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

hPMLIII (PML Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRYc-ABE8e-EGFP

Plasmid

#208340

Purpose

Expresses SpRYc-ABE8e in mammalian cells

Depositor

Pranam Chatterjee

Article

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

ecTadA(8e)-nSpRYc

Use

CRISPR

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CMV

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

Plasmid

#213969

Purpose

AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1_puro_shNRF2-2

Plasmid

#230087

Purpose

shRNA silencing human NRF2 gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-hHBEGF

Plasmid

#199234

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Proheparin-binding EGF-like growth factor (HBEGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ9714: pHR-PGK-SV40_NLS-dCasMINI-V4-NZF-c-Myc_NLS-mCherry-WPRE

Plasmid

#203220

Purpose

Expression of dCasMINI-V4-NZF-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-NZF-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTMB301_ZFcharm_Kv1_Prnp_SPM

Plasmid

#220840

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB

Plasmid

#183657

Purpose

N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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MCP-3x9aaTAD

Plasmid

#210707

Purpose

This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

N55K in MCP

Promoter

EF1a

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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HA-KIF5B*-strep

Plasmid

#120170

Purpose

Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTMB304_ZFcharm_Kv1_Prnp_SCR

Plasmid

#220842

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTP434

Plasmid

#104167

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

3x Cysteines located at bps 493-495, 520-522, and…

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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