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We narrowed to 5,403 results for: puc

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Showing: 5261 - 5280 of 5403 results

  4. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  5. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

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  7. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  10. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  11. pJW1595

    Plasmid
    #121062
    Purpose
    mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121062-plasmid-map-sequence-id-235983

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  12. INCTbiosyn-p35S-GFP(rc)13

    Plasmid
    #127526
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127526-plasmid-map-sequence-id-250841

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  14. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  15. pJW1592

    Plasmid
    #121059
    Purpose
    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    GFP^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121059-plasmid-map-sequence-id-235964

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  16. Bxb1Donor_G102H_ADRB2_LucReporter

    Plasmid
    #129794
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    G102H
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129794-plasmid-map-sequence-id-255134

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  18. pJW1594

    Plasmid
    #121061
    Purpose
    TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    TagRFP-T^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121061-plasmid-map-sequence-id-235980

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  19. pSP148

    Plasmid
    #247110
    Purpose
    Expresses estrogen-binding domain-GFP-nanobody-Rnf4 fusion protein in Aspergillus fumigatus
    Depositor
    Scott Moye-Rowley
    Article
    Paul et al G3 (Bethesda). 2026 Feb 4;16(2):jkaf295.
    Inserts
    Human estrogen-binding domain from the estrogen receptor (ESR1 Human)
    GFP Nanobody
    Rat Rnf4 (Rnf4 Rat)
    Use
    Affinity Reagent/ Antibody; Maintenance in bacter…
    Tags
    4X HA
    Expression
    Bacterial
    Available Since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    247110-plasmid-map-sequence-id-490709

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  20. TK4_SH7_targeting

    Plasmid
    #254454
    Purpose
    TK4 targeting vector containing left and right homology arms for the SH7 locus
    Depositor
    Marius Wernig
    Article
    Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2
    Inserts
    mycNLS-mScarlet-T2A-mNeonGreen-PEST
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available Since
    April 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    254454-plasmid-map-sequence-id-507705

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Showing: 5261 - 5280 of 5403 results