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pTol2-eab2-AG

Plasmid

#182155

Purpose

Expression of chimeric AG (N-mApple-intron-C-eGFP)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AG

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-GA

Plasmid

#182156

Purpose

Expression of chimeric GA (N-eGFP-intron-C-mApple)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

GA

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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bTub-GFP.766A

Plasmid

#112695

Purpose

expressed GFP in testes, under bTub promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

bTub-GFP-SV40

Expression

Insect

Promoter

bTub

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET-s34T

Plasmid

#28256

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain H3-H4 with Thrombin site

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between H3 and H4

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pTSA-38

Plasmid

#17328

Depositor

Takeshi Sano

Article

Sano et al Proc Natl Acad Sci U S A. 1995 Apr 11. 9

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available Since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
pKM744

Plasmid

#134225

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP T + CENP W

Expression

Insect

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMVS223

Plasmid

#176293

Purpose

SyntheticTranscriptionFactor_loxFAS_PGK_MCS_scarlett_ERD_ZIFDBD_P2A_Neo_loxP_AMP

Depositor

Max Staller

Article

Staller et al Cell Syst. 2022 Apr 20;13(4):334-345.e5.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-I70V

Plasmid

#47663

Purpose

pAC-EsaR with a208g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
myc-NLRC5 deltaDD

Plasmid

#37511

Depositor

Thomas Kufer

Article

Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

Insert

NLRC5 (NLRC5 Human)

Tags

myc

Expression

Mammalian

Mutation

lacking the N-terminal domain (deletion: aa 1-134)

Promoter

CMV

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pMT-GFP-SAK

Plasmid

#31755

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Sak (SAK Fly)

Tags

GFP

Expression

Insect

Mutation

A115S

Promoter

Metallothionein

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
CJ54 (cg5166a/c utr mut)

Plasmid

#17782

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg5166a/c utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pMT-GFP-Sas4

Plasmid

#31756

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Sas4 (Sas-4 Fly)

Tags

GFP

Expression

Insect

Mutation

N70T

Promoter

Metallothionein

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pLUM

Plasmid

#64169

Purpose

harbours marker genes LEU2, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTH646-2µ-RLuc

Plasmid

#40600

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pYC12

Plasmid

#63911

Purpose

Initial Overexpressing Vector

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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BII-ChPtinGW

Plasmid

#133377

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-che-DAS+4

Plasmid

#27060

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-mcherry rfp-DAS+4

Expression

Bacterial

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pQCXP CMV/TO V5-LUC (w476-1)

Plasmid

#17740

Purpose

Retroviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Luciferase and Retroviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-bat-Caspase-4

Plasmid

#183377

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRC05

Plasmid

#114684

Purpose

FKBP-TEV-C

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FKBP-TEV-C

Tags

GST

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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S019

Plasmid

#174552

Purpose

pET-iBclxL-v3, His tag for affinity purification

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

iBclxL-v3, Bclxl inhibitor resistant protein

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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S330

Plasmid

#174553

Purpose

pET-iBcl2-v4, His tag for affinity purification

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

ibcl2-v4, bcl2 inhibitor resistant protein

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGMEH-P38-che-ssrAec3

Plasmid

#27059

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-mcherry rfp-ssrAec

Expression

Bacterial

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pMEP5-Flag Par6 WT

Plasmid

#24647

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

Par6 (Pard6a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBALU6

Plasmid

#69321

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: Venus in frame w/ FRT-galK-FRT; no introns; N-terminal fusion

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

Venus-FgF

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pDS255

Plasmid

#34970

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pRC04

Plasmid

#114683

Purpose

FRB-TEV-N

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FRB-TEV-N

Tags

MBP

Expression

Bacterial

Promoter

Tac Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-Cre.ape

Plasmid

#71749

Purpose

U+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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CS2 luc Utrn

Plasmid

#13500

Depositor

Stephen Tapscott

Article

Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

Insert

Utrophin (Utrn Mouse)

Use

Luciferase

Mutation

Contains 5 copies of nt 10657-10693 of NM_011682.

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

Plasmid

#58522

Purpose

Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pBN281

Plasmid

#86726

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Plin-31::FLP D5

Expression

Worm

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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CDS12_mScarlet-I

Plasmid

#136087

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mScarletI(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMPRMA38

Plasmid

#172937

Purpose

pQE30 encoding Wzz mutant i92

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i92 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA45

Plasmid

#172934

Purpose

pQE30 encoding Wzz mutant i32

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i32 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA48

Plasmid

#172941

Purpose

pQE30 encoding Wzz mutant i131

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i131 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPRMA50

Plasmid

#172936

Purpose

pQE30 encoding Wzz mutant i219

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i219 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN258

Plasmid

#86721

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Punc-119::FLP D5

Expression

Worm

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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CMVTO-Fyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp

Plasmid

#179668

Purpose

Membrane-bound (Fyn) TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTlpAreverse-Wasabi

Plasmid

#86114

Purpose

mWasabi reporter gene driven by TlpA promoter in reverse orientation

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA (in reverse orientation)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0623

Plasmid

#128998

Purpose

pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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