We narrowed to 14,583 results for: otos

1. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

   Plasmid

   #58490

   Purpose

   Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3

   Insert

   Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Aspartic Acid 95 to Glutamine

   Promoter

   Ubiquitin

   Available since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. XE159 GFP-delta LZ-XDpr-delta PDZB-CS2+

   Plasmid

   #16763

   Depositor

   Randall Moon

   Article

   Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

   Insert

   delta LZ-XDpr-delta PDZB (dact1-b Frog)
   

   Use

   Xenopus expression

   Tags

   gfp

   Expression

   Mutation

   XDpr that is doubly truncated to delete the first…

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pET28aAviHisCD72aCTLD

   Plasmid

   #129065

   Purpose

   generation of biotinylated mouse CD72a CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Article

   Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

   Insert

   CD72a (Cd72 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3 CrkII

   Plasmid

   #50731

   Purpose

   mammalian expression of CrkII

   Depositor

   Kenneth Yamada

   Article

   Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

   Insert

   CrkII (CRK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pJL017

   Plasmid

   #53532

   Purpose

   This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpBA
   

   Use

   Tags

   6His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMV306hsp+LuxG13

   Plasmid

   #26161

   Depositor

   Brian Robertson, Siouxsie Wiles

   Article

   Andreu et al PLoS One. 2010 May 24;5(5):e10777.

   Insert

   Bacterial luciferase operon + G13 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   It contains a gram-positive enhanced translation …

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGM3B

   Plasmid

   #110809

   Purpose

   contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes

   Depositor

   David Russell

   Article

   Khan et al Nat Protoc. 2011 Apr;6(4):482-501. doi:

   Insert

   AAV3B cap genes, AAV2 rep genes, adenovirus helper genes
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRXIP-3xMyc-STX17FL

   Plasmid

   #89942

   Purpose

   Expresses syntaxin17 tagged with Myc in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

   Insert

   human syntaxin17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Avr2-GFP pZK538

    Plasmid

    #118011

    Purpose

    expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .

    Depositor

    FLW Takken

    Article

    et al Bio-protocol 8(24): e3114.

    Inserts

    dspAvr2
    mCherry
    

    Use

    Tags

    EGFP and HDEL

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. murine TCR OTII-2A.pMIG II

    Plasmid

    #52112

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    P2A (from porcine teschovirus-1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-NES-PhoCl-mCherry

    Plasmid

    #87690

    Purpose

    Express NES-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NES-PhoCl-mCherry
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE_puro

    Plasmid

    #118692

    Purpose

    Empty vector to express overexpression cDNA

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-luciferase-P2A-Neo

    Plasmid

    #105621

    Purpose

    3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

    Plasmid

    #87693

    Purpose

    Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-P-MCS

    Plasmid

    #80488

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiPGK DEST H2B-iRFP670

    Plasmid

    #90237

    Purpose

    Lentiviral vector to express H2B-iRFP670 under PGK promoter

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    H2B
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-H2B-GFP

    Plasmid

    #116869

    Purpose

    Nuclear localized GFP

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGs-Foxp1

    Plasmid

    #45720

    Depositor

    Jeremy Dasen

    Article

    Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

    Insert

    FoxP1 (Foxp1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

20. EGFP-Tubulin-6

    Plasmid

    #56450

    Purpose

    Localization: Microtubules, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pTALdNC-AAVS1_T1

    Plasmid

    #80495

    Purpose

    AAVS1 TALEN expression vector (Left)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Left)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTALdNC-AAVS1_T2

    Plasmid

    #80496

    Purpose

    AAVS1 TALEN expression vector (Right)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Right)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-myc3-CUL4A

    Plasmid

    #19951

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CUL4A (cullin 4A) (CUL4A Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3 myc CIITA (P#808)

    Plasmid

    #14650

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

    Insert

    CIITA (CIITA Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMD.RVG.CVS24-B2c

    Plasmid

    #19713

    Depositor

    Manfred Schubert

    Article

    Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

    Insert

    Rabies virus CVS24-B2c glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMD.RVG.CVS24-N2c

    Plasmid

    #19712

    Depositor

    Manfred Schubert

    Article

    Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

    Insert

    Rabies virus CVS24-N2c glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

28. Lenti-Hb9-GFP

    Plasmid

    #37080

    Purpose

    3rd generation lentiviral transfer plasmid for Hb9 GFP

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Hb9

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hBACCS2

    Plasmid

    #67625

    Purpose

    Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    hBACCS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-H2B tdTomato

    Plasmid

    #116870

    Purpose

    Nuclear localized tdTomato

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    H2B tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge